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目的 研究α-淀粉酶与致龋细菌粘附的关系,探讨α-淀粉酶与龋病的相关性。方法 采用不同浓度α-淀粉酶及全唾液作为实验性获得性膜成分,观察经放射性同位素3H-TDR标记了的变形链球菌、血链球菌对羟基磷灰石的粘附情况。结果（1）变形链球菌的粘附量显著大于血链球菌（P<0.05）;（2）与对照组相比,α-淀粉酶能明显抑制变形链球菌、促进血链球菌对羟基磷灰石的粘附,而且其作用均随浓度增加而增强（P<0.05）;（3）α-淀粉酶组与无龋正常人全唾液组相比,对血链球菌粘附的作用无区别（P>0.05）,对变形链球菌粘附的作用因α-淀粉酶浓度不同而有差异。结论α-淀粉酶与龋病的发生、发展可能有直接关系。
Objective To study the relationship between α-amylase and cariogenic bacteria adhesion and to explore the relationship between α-amylase and caries. Methods Different concentrations of α-amylase and whole saliva were used as experimental membrane components to observe the adhesion of Streptococcus mutans and Streptococcus sanguis to hydroxyapatite labeled with radioisotope 3H-TDR. Results (1) Streptococcus mutans adhesion was significantly greater than that of Streptococcus sanguis (P <0.05); (2) Compared with the control group, α-amylase could significantly inhibit Streptococcus mutans and promote Streptococcus sanguis against hydroxyapatite (P <0.05). (3) There was no difference between the α-amylase group and the carotid-free normal whole saliva group on the adhesion of Streptococcus sobrinus P> 0.05), the role of Streptococcus mutans adhesion due to different concentrations of alpha-amylase have differences. Conclusion α-amylase may be directly related to the occurrence and development of dental caries.