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目的:急性心肌梗死(acute myocardial infraction,AMI)患者外周血中血管紧张素Ⅱ(angiotensinⅡ,Ang-Ⅱ)水平对内皮祖细胞(endothelial progenitor cells,EPCs)的数目变化的影响。方法:入选的28例AMI患者(实验组)和30例冠状动脉造影阴性患者(对照组),用ELISA法检测AMI患者急性期、恢复期以及对照组血清中Ang-Ⅱ、基质细胞衍生因子-1α(stromal cell-derived factor-1α,SDF-1α)的水平,同时用密度梯度离心法获得单个核细胞,用CD34、VEGF和CD133抗体标定EPCs,用流式细胞仪检测外周血中EPCs占单个核细胞的比率。结果:AMI患者急性期Ang-Ⅱ水平(90.40±6.39)pg/ml明显高于恢复期(72.57±2.42)pg/ml和对照组(69.14±3.73)pg/ml(P=0.002 4),同时AMI患者急性期EPCs占外周血单个核细胞的比率为为(0.043±0.004)%,明显高于恢复期(0.024±0.010)%和对照组(0.012±0.001)%(P<0.000 1),但AMI患者急性期SDF-1α的水平(171.1±35.81)pg/ml同恢复期(130.30±28.84)pg/ml及对照组(116.50±29.19)pg/ml之间无明显差异(P=0.370 3)。在AMI患者急性期外周血中EPCs和Ang-Ⅱ水平呈负相关,而与SDF-1α水平无明显相关性。结论:AMI患者急性期循环中的Ang-Ⅱ水平及EPCs数量均升高,但是Ang-Ⅱ抑制了EPCs的数量。
Objective: To investigate the effect of angiotensin Ⅱ (Ang-Ⅱ) on the number of endothelial progenitor cells (EPCs) in peripheral blood of patients with acute myocardial infraction (AMI). Methods: A total of 28 patients with AMI (experimental group) and 30 patients with negative angiography (control group) were enrolled in this study. The serum levels of Ang-Ⅱ and stromal cell derived factor - 1α (SDF-1α). Meanwhile, mononuclear cells were obtained by density gradient centrifugation. EPCs were labeled with CD34, VEGF and CD133 antibodies. Flow cytometry was used to detect the percentage of EPCs in peripheral blood The ratio of nuclear cells. Results: The Ang Ⅱ level (90.40 ± 6.39) pg / ml in AMI patients was significantly higher than that in recovery patients (72.57 ± 2.42 pg / ml vs 69.14 ± 3.73 pg / ml, P = 0.002 4) The ratio of EPCs to peripheral blood mononuclear cells in AMI patients was (0.043 ± 0.004)% at acute stage, significantly higher than that at recovery (0.024 ± 0.010)% and control group (0.012 ± 0.001)% (P <0.000 1) There was no significant difference (P = 0.370 3) between the level of SDF-1α (171.1 ± 35.81) pg / ml and the recovery phase (130.30 ± 28.84) pg / ml and the control group (116.50 ± 29.19 pg / . There was a negative correlation between the levels of EPCs and Ang-Ⅱ in acute peripheral blood of patients with AMI and no significant correlation with the level of SDF-1α. CONCLUSIONS: The levels of Ang-Ⅱ and the number of EPCs in acute phase of AMI patients are increased, but Ang-Ⅱ inhibits the number of EPCs.