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目的观察出生后早期有限次数速灭威染毒对ICR小鼠青春期和成年早期神经行为和空间学习记忆能力的影响以及自主活动能力、感觉运动能力和焦虑情绪是否影响空间学习记忆能力,并探明其影响的发生时间。方法小鼠出生后随机分为5组,即速灭威高、中、低剂量组(5 mg/kg、0.5 mg/kg、0.05 mg/kg,),溶剂对照组(DMSO)和生理盐水对照组(NS),于出生后第3、5、7、9、11、13天进行腹腔注射染毒。观察一般生理发育情况,并选用成组的神经行为学实验在青春期(～PND60)和成年早期(～PND90)进行测试。结果青春期小鼠平衡木得分与染毒剂量呈负相关关系(rs=-0.418,P<0.05);青春期和成年早期的洞板实验和旷场实验中各指标在同期各组间均无统计学意义;青春期Morris水迷宫空间探索实验中,定位航行结束后和次日的两次穿越平台位置次数与染毒剂量之间均呈负相关关系(rs分别为-0.361和-0.277,P<0.05);成年早期Morris水迷宫空间探索实验中,第四象限时间百分比与染毒剂量之间呈负相关关系(rs=-0.390,P<0.05);主成分分析发现,反映空间学习记忆能力与自主活动能力、感觉运动能力及焦虑情绪的指标出现在不同的主成分中。结论出生后早期有限次数接触速灭威可能在青春期时已经开始影响到小鼠的空间记忆能力,这可能是成年后空间学习记忆能力损害的重要线索。
OBJECTIVE: To observe the effects of arsenopyrite on the neurobehavioral, spatial learning and memory abilities of adolescents and early adolescents in ICR mice and the effects of autonomic motor activity, sensory motor ability and anxiety on spatial learning and memory ability The timing of its impact. Methods The mice were randomly divided into 5 groups after birth, namely the fasting high, medium and low dose groups (5 mg / kg, 0.5 mg / kg, 0.05 mg / kg), solvent control group (DMSO) and saline control Group (NS), were injected intraperitoneally on the 3rd, 5th, 7th, 9th, 11th and 13th day after birth. Observed the general physiological development, and the use of a group of neurobehavioral experiments in adolescence (~ PND60) and early adulthood (~ PND90) were tested. Results There was a negative correlation between balance beam score and exposure dose in adolescent mice (rs = -0.418, P <0.05). There was no significant difference between adolescent and adolescent cave-board experiments and open field during the same period In adolescent Morris water maze space exploration experiment, there was a negative correlation between the number of platform positions and the dose of exposure after the positioning navigation and the next day (rs = -0.361 and -0.277, P <0.05, respectively). In the experiment of spatial exploration of Morris water maze in early age, there was a negative correlation between the percentage of time in the fourth quadrant and exposure dose (rs = -0.390, P <0.05). The principal component analysis showed that there was a negative correlation between spatial learning and memory ability and autonomic activity , Sensory motor ability and anxiety indicators appear in different main components. Conclusions A limited number of exposure to metomycin at the early postnatal period may have begun to affect spatial memory in mice during adolescence, which may be an important clue to the impairment of spatial learning and memory in adulthood.