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In this study,PUP-type cytokinin transporter genes were identifed in rice(Oryza sativa L.).The Oryza sativa purine permease(OsPUP)family has 12 members that show similar predicted protein sequences with AtPUPs.To reveal the functions of OsPUP genes,we searched the T-DNA mutant library of rice and found one mutant for the member OsPUP7.The T-DNA insertion caused a new transcript that encodes a protein with 26 amino acids different from the native OsPUP7 at the C-terminus.The mutant showed multiple phenotypic changes including increased plant height,big seeds,and delayed fowering.The mutant also showed increased sensitivity to drought and salt stresses and treatments with kinetin and abscisic acid.OsPUP7 is expressed mainly in the vascular bundle,pistil,and stamens.The measurement of cytokinins(CKs)showed that CK content in the mutant spikelets accumulated higher than that in the wild type.Moreover,uptake experiment in the yeast fcy2 mutant suggested that OsPUP7 has the ability to transport caffeine,a CK derivative.Our results indicate that the PUP transport system also exists in rice,and OsPUP7 has an important role in the transport of CK,thus affecting developmental process and stress responses.
In this study, PUP-type cytokinin transporter genes were identified in rice (Oryza sativa L.). The Oryza sativa purine permease (OsPUP) family has 12 members that show similar predicted protein sequences with AtPUPs.To reveal the functions of OsPUP genes, we searched the T-DNA mutant library of rice and found one mutant for the member OsPUP7. The T-DNA insertion caused a new transcript that encodes a protein with 26 amino acids different from the native OsPUP7 at the C-terminus. multiple phenotypic changes including increased plant height, big seeds, and delayed fowering. The mutant also showed increased sensitivity to drought and salt stresses and treatments with kinetin and abscisic acid. OsPUP7 is expressed mainly in the vascular bundle, pistil, and stamens. measurement of cytokinins (CKs) showed that CK content in the mutant spikelets accumulated higher than that in the wild type. More than, upper take experiment in the yeast fcy2 mutant suggested that OsPUP7 has the ability to transp ort caffeine, a CK derivative. Our results that that the PUP transport system also exists in rice, and OsPUP7 has an important role in the transport of CK, thus affecting developmental process and stress responses.