革兰氏染色法是对细菌染色最常用的一种特殊染色方法,它是一种退行性染色法,是染色后通过分化剂处理能保持着色的一类细菌,染成蓝紫色,称为革兰氏阳性细菌,而通过分化剂处理完全脱色的一类细菌,不着色,则称为革兰氏阴性细菌。其操作简单、快捷、成本低,对病变病因学的研究有重要作用。但革兰氏染色法多用于细胞涂片和菌落涂片,在石蜡切片很少使用,我们在日常病理工作中,运用革兰氏 Gram staining is the most common staining method for bacteria, a special staining method. It is a degenerative staining method. It is a kind of bacteria that can maintain the coloring after being treated by the differentiation agent and is dyed blue-purple, called leather Lan positive bacteria, and through the differentiation agent to deal with completely decolorization of a class of bacteria, non-colored, then called Gram-negative bacteria. The operation is simple, fast, low cost, the etiology of the disease has an important role in the study. However, Gram staining is often used in cell smears and colony smears, rarely used in paraffin sections, we use the routine pathology, the use of Gram