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本文采用一步法制备了1-芘丁酸/石墨烯复合物(PBA/G),并研究了它的电化学性质.采用铁氰化钾和亚铁氰化钾电化学探针测定了其电化学阻抗滴定曲线,确定了PBA/G的表观pKa为6.2.此外,将葡萄糖氧化酶(GOD)共价键合在PBA/G表面构建了葡萄糖电化学传感器,其电化学响应与葡萄糖浓度(5 mmol.L-1浓度范围内)呈线性关系,检测限为0.085 mmol.L-1.实验还测定了固定在PBA/G表面的GOD的表观米氏常数为5.40mmol.L-1,表明固定化的GOD对葡萄糖有较高的催化活性.
In this paper, the 1-pyrene butyric acid / graphene composite (PBA / G) was prepared by one-step method and the electrochemical properties of PBA / G were studied. The electrochemical properties of PBA / G were investigated by electrochemical probe of potassium ferricyanide and potassium ferrocyanide Chemical impedance titration curve, the apparent pKa of PBA / G was determined to be 6.2. In addition, a glucose electrochemical sensor was constructed by covalently bonding glucose oxidase (GOD) to the surface of PBA / G, and its electrochemical response was correlated with the concentration of glucose 5 mmol·L-1), the detection limit was 0.085 mmol·L-1. The apparent Michaelis constant of GOD immobilized on the surface of PBA / G was also determined to be 5.40 mmol·L-1, The immobilized GOD showed higher catalytic activity on glucose.