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粘多糖是由糖醛酸和氨基己糖交替连接成的高分子物质,理化性质独特,应用范围广泛。通过对突变株兽疫链球菌Streptococcus zooepidemicus BU100进行发酵,可产一种新型粘多糖(下文用粘多糖A代替)。利用咔唑法、Elson-Morgan法、考马斯亮蓝法、红外光谱以及13C核磁共振谱测定粘多糖A的结构,结果显示粘多糖A中糖醛酸和氨基糖的摩尔比例接近1:1,蛋白含量符合标准(<0.1%);粘多糖A图谱中出现的结构特征峰大部分与透明质酸相同。对粘多糖A的实用性能进行检测,并用透明质酸做对比,结果表明透明质酸在两种湿度下的吸湿性均要好于粘多糖A,但粘多糖A的保湿性要好于透明质酸。粘多糖A总体的抗氧化性好于透明质酸,并且粘多糖A耐透明质酸酶。粘多糖A可作为保湿剂、润滑剂、抗氧化剂等被更加有效地应用在医疗和化妆品等领域。
Mucopolysaccharidose is a kind of macromolecule material which is formed by alternating uronic acid and hexose hexose, which has unique physical and chemical properties and wide range of applications. By fermentation of the mutant strain Streptococcus zooepidemicus BU100, a new type of mucopolysaccharide (hereinafter, replaced with mucopolysaccharide A) can be produced. The structure of mucopolysaccharide A was determined by carbazole method, Elson-Morgan method, Coomassie brilliant blue method, infrared spectroscopy and 13C nuclear magnetic resonance spectroscopy. The results showed that the molar ratio of uronic acid to amino sugar in mucopolysaccharide A was close to 1: 1, The content was in line with the standard (<0.1%); most of the structural characteristic peaks appearing in the mucopolysaccharide A spectrum were the same as hyaluronic acid. The practical performance of mucopolysaccharide A was tested and compared with hyaluronic acid. The results showed that the hygroscopicity of hyaluronic acid was better than that of mucopolysaccharide A in both humidity, but mucopolysaccharide A had better moisture retention than hyaluronic acid. The overall anti-oxidant properties of mucopolysaccharide A is better than that of hyaluronic acid, and mucopolysaccharide A is resistant to hyaluronidase. The mucopolysaccharide A is more effectively used as a moisturizer, a lubricant, an antioxidant, and the like in fields such as medical treatment and cosmetics.