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目的探讨拓扑异构酶Ⅱα(TopoⅡα)和PTEN蛋白在卵巢上皮性癌组织中的表达及与临床病理特征的关系。方法应用免疫组化法检测56例卵巢上皮性癌组织,30例良性卵巢肿瘤及15例正常卵巢组织中TopoⅡα和PTEN蛋白的表达。结果 (1)TopoⅡα在良性卵巢肿瘤组织及正常卵巢组织中的阳性表达率为16.7%、13.3%,显著低卵巢癌组织的53.6%,差异有显著性(P<0.05);TopoⅡα表达与卵巢癌临床分期、淋巴结转移有关,而与年龄、病理类型、组织学分级无关。(2)PTEN蛋白在卵巢癌组织中的阳性表达率为33.9%,低于正常卵巢和良性上皮肿瘤组织的93.3%,100%(P<0.05);PTEN蛋白表达与卵巢癌临床分期、组织学分级、淋巴结转移有关,而与年龄、病理类型无关。(3)卵巢癌组织中TopoⅡα和PTEN蛋白间的表达呈负相关(r=-0.507,P<0.05)。结论 TopoⅡα和PTEN蛋白的异常表达可能与上皮性卵巢癌的发生、发展、侵袭性和预后相关,通过对它们的检测可以对卵巢癌的预后及化疗提供理论依据。
Objective To investigate the expression of topoisomerase Ⅱα (TopoⅡα) and PTEN protein in epithelial ovarian cancer and its relationship with clinicopathological features. Methods The expression of TopoⅡα and PTEN protein in 56 cases of epithelial ovarian cancer, 30 cases of benign ovarian tumor and 15 cases of normal ovarian tissue were detected by immunohistochemistry. Results (1) The positive rate of TopoⅡα in benign ovarian tumor tissues and normal ovarian tissues was 16.7%, 13.3%, significantly lower than that in ovarian cancer tissues 53.6% (P <0.05). The expression of TopoⅡα in ovarian cancer Clinical stage, lymph node metastasis, but not with age, pathological type, histological grade unrelated. (2) The positive expression rate of PTEN protein in ovarian cancer tissues was 33.9%, which was lower than that in normal ovarian tissues and benign epithelial tumor tissues (93.3%, 100%, P <0.05) Grade, lymph node metastasis, but not with the age, type of pathology. (3) There was a negative correlation between the expression of TopoⅡα and PTEN protein in ovarian cancer (r = -0.507, P <0.05). Conclusion The abnormal expression of TopoⅡα and PTEN protein may be related to the occurrence, development, invasion and prognosis of epithelial ovarian cancer. The detection of them can provide a theoretical basis for the prognosis and chemotherapy of ovarian cancer.