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目的:观察圣愈汤对骨髓抑制小鼠外周血、骨髓细胞周期和凋亡的影响,并探讨其造血调控的可能机制。方法:将60只BALB/c小鼠随机分为空白组、模型组、阳性药组、圣愈汤32,16,8 g.kg-1.d-1 3个剂量组,每组各10只,除空白组外,其他5组用60Coγ照射和ip注射环磷酰胺、氯霉素制备骨髓抑制小鼠模型。造模完成24 h后,空白组和模型组小鼠ig生理盐水,圣愈汤三剂量组分别按含生药32,16,8 g.kg-1.d-1ig给药,给药体积20 mL.kg-1.d-1;阳性组ip rhG-CSF 125μg.kg-1.d-1,各组均连续给药7 d。用全自动血细胞分析仪、白细胞计数法、流式细胞术(FCM)分别检测圣愈汤对骨髓抑制小鼠外周血、骨髓有核细胞数、骨髓细胞周期和凋亡率的影响。结果:圣愈汤32,16 g.kg-1.d-1均能明显升高外周血白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)、血小板(PLT)和骨髓有核细胞(BMC)数(P<0.05或P<0.01)。模型组、圣愈汤32,16 g.kg-1.d-1组G0/G1期细胞比例分别为(70.38±1.38)%,(62.43±2.03)%,(63.85±2.90)%;S期比例分别为(22.52±1.27)%,(26.35±2.52)%,(24.80±1.48)%;G2/M期细胞比例(7.43±1.04)%,(11.37±2.18)%,(10.95±2.35)%;PI分别为(29.85±1.06)%,(37.66±2.01)%,(35.88±3.13)%;凋亡率分别为(26.88±3.58)%,(19.15±4.30)%,(21.65±4.34)%。圣愈汤32,16g.kg-1.d-1组与模型组比,上述指标有显著差异(P<0.05或P<0.01)。结论:圣愈汤能改善骨髓抑制小鼠的造血机能,其机制之一可能是通过促进骨髓造血细胞进入细胞增殖周期和抑制其凋亡。
Objective: To observe the effects of Shengyu Decoction on peripheral blood and bone marrow cell cycle and apoptosis in myelosuppressed mice, and to explore the possible mechanism of hematopoietic regulation. Methods: Sixty BALB / c mice were randomly divided into three groups: blank group, model group, positive drug group and Shengyu Decoction 32,16,8 g.kg-1.d-1, with 10 mice in each group , Except the blank group, the other five groups were treated with 60Coγ irradiation and ip injection of cyclophosphamide, chloramphenicol myelosuppression mice model. After 24 h of modeling, the mice in the blank group and the model group were treated with ig saline and Shengyu Decoction three doses respectively at doses of 32, 16 and 8 g.kg-1.d-1ig, .kg-1.d-1; positive group ip rhG-CSF 125μg.kg-1.d-1, each group were administered for 7 days. The effects of Shengyu Decoction on the peripheral blood, the number of nucleated bone marrow cells, the bone marrow cell cycle and the apoptosis rate of myelosuppressed mice were detected by automatic hematology analyzer, leukocyte counting method and flow cytometry (FCM) respectively. Results: Shengyu Decoction 32,16 g.kg-1.d-1 can significantly increase the peripheral blood leukocytes (WBC), red blood cells (RBC), hemoglobin (Hb), platelets (PLT) and bone marrow nucleated cells ) (P <0.05 or P <0.01). (70.38 ± 1.38)%, (62.43 ± 2.03)%, (63.85 ± 2.90)% respectively in the model group and Shengyu Decoction 32,16 g.kg-1.d-1 group (22.52 ± 1.27)%, (26.35 ± 2.52)% and (24.80 ± 1.48)%, respectively. The proportion of cells in G2 / M phase was (7.43 ± 1.04)%, (11.37 ± 2.18)% and ; The PI were respectively (29.85 ± 1.06)%, (37.66 ± 2.01)% and (35.88 ± 3.13)%; the apoptotic rates were (26.88 ± 3.58)%, (19.15 ± 4.30)% and (21.65 ± 4.34) . Sheng Yu Tang 32,16g.kg-1.d-1 group and the model group, the above indicators were significantly different (P <0.05 or P <0.01). Conclusion: Shengyu Decoction can improve the hematopoietic function in myelosuppressed mice. One of the mechanisms may be through promoting the proliferation of bone marrow hematopoietic cells into the cell cycle and inhibiting the apoptosis.