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目的观察刺五加提取物中总黄酮的含量及其对红细胞膜流动性的影响。方法采用分光光度法测定刺五加总黄酮的含量,采用荧光偏振法测定红细胞膜流动性和微粘度。结果刺五加提取物与刺五加注射液的总黄酮含量相近,两组溶液均可增加红细胞膜流动性,降低微粘度,其中刺五加注射液的作用更为显著。结论刺五加总黄酮可以增加红细胞膜流动性,从而降低血液粘度,改善血液流变学指标。
Objective To observe the content of total flavonoids in Acanthopanax senticosus extract and its effect on the erythrocyte membrane fluidity. Methods The content of total flavonoids in Acanthopanax senticosus was determined by spectrophotometry, and the mobility and microviscosity of red blood cell membrane were measured by fluorescence polarization method. Results The contents of total flavonoids of Acanthopanax senticosus extract and Acanthopanax injection were similar. Both of the two solutions could increase the fluidity of red blood cell membrane and reduce the micro viscosity. Among them, the effect of Acanthopanax injection was more significant. Conclusion The total flavonoids of Acanthopanax senticosus can increase the erythrocyte membrane fluidity, thus reducing the blood viscosity and improving the hemorheology indexes.