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目的:探讨N-乙酰半胱氨酸(NAC)对大鼠肾草酸钙结石形成的影响及机制,为临床预防尿路结石提供理论依据。方法:将30只健康清洁成年雄性Wistar大鼠先在相同环境下适应性喂养1周,然后随机分为3组:A组(空白对照组)、B组(单纯诱石组)、C组(诱石+NAC干预组)。A组饮去离子水,B组饮1%乙二醇的去离子水,C组饮1%乙二醇去离子水,并给予NAC 100mg/(kg.d)灌胃。第4周处死大鼠,取出双肾,左肾纵向剖开,用10%甲醛固定,HE染色石蜡切片,在100、400倍光镜下观察肾组织草酸钙结晶沉积情况,并进行分级及评分。右肾皮质制成10%的匀浆,检测丙二醛(MDA)及超氧化物歧化酶(SOD)。全部实验数据通过SPSS17.0统计软件分析处理。结果:①肾脏结晶沉积情况分级及评分结果:与B组相比,C组的肾脏结晶沉积评分明显降低(P<0.05)。②MDA检测结果:与B组相比,C组的MDA含量降低,差异有统计学意义(P<0.05)。③SOD检测结果:与B组相比,C组的SOD含量增高,差异有统计学意义(P<0.05)。④A、B、C三组肾组织结晶等级评分与SOD含量的相关系数为-0.499(P<0.01),结晶评分与MDA含量的相关系数为0.592(P<0.01)。结论:NAC可以通过其抗氧化作用抑制大鼠肾草酸钙结石的形成。
Objective: To investigate the effect and mechanism of NAC on the formation of renal calcium oxalate stones in rats and provide a theoretical basis for clinical prevention of urolithiasis. Methods: Thirty healthy male adult Wistar rats were fed for 1 week under the same conditions and then randomly divided into 3 groups: group A (blank control group), group B (simple lure group), group C Lure + NAC intervention group). Group A drank deionized water, group B drank 1% ethylene glycol deionized water, group C drank 1% ethylene glycol deionized water, and given NAC 100mg / (kg.d) gavage. The rats were sacrificed on the fourth week, the kidneys were removed, the left kidney was dissected longitudinally, fixed with 10% formaldehyde, and stained with HE for paraffin sections. The deposition of calcium oxalate crystals in kidney tissues was observed under 100,400 times light microscope and graded and scored . Right renal cortex made of 10% homogenate, malondialdehyde (MDA) and superoxide dismutase (SOD) were detected. All experimental data by SPSS17.0 statistical software analysis and processing. Results: ① Grading of renal deposits and grading results: Compared with group B, the deposition of renal deposits in group C decreased significantly (P <0.05). ②MDA test results: compared with the B group, C group MDA content decreased, the difference was statistically significant (P <0.05). ③ SOD detection results: Compared with B group, C group SOD increased, the difference was statistically significant (P <0.05). ④ The correlation coefficient between the grade of renal tissue and SOD in group A, B and C was -0.499 (P <0.01), and the correlation coefficient between the score of crystallization and the content of MDA was 0.592 (P <0.01). Conclusion: NAC can inhibit the formation of renal calcium oxalate stones in rats through its antioxidation.