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目的 探讨交链孢酚 (AOH)诱导食管癌的发生机制。方法 用免疫组化ABC法分别对AOH处理 2 4h、1周和 3周的胎儿食管上皮组织及原发性食管癌癌变各阶段组织进行P5 3蛋白表达的检测。结果 AOH诱导 1周的胎儿食管上皮中即有P5 3蛋白过表达 ,并一直持续到第 3周 ;食管癌变的早期阶段出现P5 3蛋白的聚集 ,并随病变加重 ,P5 3阳性率呈逐渐升高趋势。结论 AOH可使P5 3蛋白表达增强 ,P5 3蛋白的高表达可出现在食管癌发生的早期阶段 ,并可能在食管癌发生发展中起重要作用
Objective To investigate the mechanism of carotenoid (AOH) -induced esophageal cancer. Methods Immunohistochemical ABC method was used to detect the expression of P53 protein in the tissues of fetal esophageal epithelium and the carcinogenesis of primary esophageal cancer at 24 hours, 1 week and 3 weeks after AOH treatment. Results P5 3 protein was overexpressed in fetal esophageal epithelium induced by AOH for 1 week and continued until the third week. P5 3 protein clustered in the early stages of esophageal carcinogenesis and gradually increased with the increase of the lesion. High trend. Conclusions AOH can enhance the expression of P53 protein. The high expression of P53 protein may appear in the early stage of esophageal carcinogenesis and may play an important role in the development of esophageal carcinoma