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目的:采用冷冻-解冻物理交联方法制备聚乙烯醇(PVA)和葡聚糖(Dextran)混合凝胶。并考察复合凝胶药物释放规律。方法:含有胰岛素的PVA/Dextran溶液置于-20℃条件下冷冻8h,在室温下解冻4h,反复冷冻-解冻数次制备得到胰岛素PVA/Dextran复合凝胶,反相高效液相色谱法考察了胰岛素的体外释放行为。结果:胰岛素复合凝胶体外释放模式符合Higuchi扩散方程。当PVA/Dextran比例从100/0降到80/20,胰岛素释放速率明显增加,最大释放率从45%增加到65%。当冷冻-解冻循环次数从2增加到6,胰岛素达到最大释放的时间从24h增加到45h,最大释放率从70%降到50%。释放介质的温度的升高能显著增加胰岛素的释放速率。结论:PVA/Dextran制备的胰岛素复合凝胶具有良好的缓释效果。PVA/Dextran复合凝胶是一种较有前景的水凝胶药物载体。
OBJECTIVE: To prepare polyvinyl alcohol (PVA) and dextran gel by freeze-thaw physical crosslinking. And investigate the law of compound gel drug release. Methods: Insulin-containing PVA / Dextran solution was frozen at -20 ℃ for 8h, thawed at room temperature for 4h and repeatedly frozen-thawed to prepare insulin / Dextran composite gel. The results of RP-HPLC Insulin in vitro release behavior. RESULTS: The in vitro release patterns of insulin composite gel were in accordance with the Higuchi diffusion equation. When the ratio of PVA / Dextran decreased from 100/0 to 80/20, the rate of insulin release increased significantly and the maximum release rate increased from 45% to 65%. When the number of freeze-thaw cycles increased from 2 to 6, the maximum release of insulin was increased from 24 h to 45 h and the maximum release rate was reduced from 70% to 50%. Increasing the temperature of the release medium can significantly increase the release rate of insulin. Conclusion: The insulin composite gel prepared by PVA / Dextran has a good sustained release effect. PVA / Dextran composite gel is a more promising hydrogel drug carrier.