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目的观察褪黑素(Melatonin,MT)对谷氨酸钠(Glutamate,Glu)致痫大鼠海马5-羟色胺(5-hydroxytryptamine,5-HT)水平的影响,研究其抑制癫痫的作用机制。方法40只健康雄性SD大鼠随机分为4组(每组10只),分别为生理盐水对照组(NS组);谷氨酸钠致痫组(Glu组);褪黑素+谷氨酸钠组(MT+Glu组);Luzidole+褪黑素+谷氨酸钠组(Luz+MT+Glu组)。观察并记录大鼠行为学及脑电图改变,用免疫组织化学方法检测大鼠海马内5-HT含量变化。结果行为学观察和EEG显示,NS组无痫样发作和痫样放电,Glu组和Luz+MT+Glu组痫样发作重(Ⅲ-Ⅴ级),脑电图显示频发高幅的痫样波,TM+Glu组无或仅有轻微发作(0-Ⅱ级),脑电图上无或偶见散在单个微小痫样波;免疫组织化学分析结果显示,Glu组和Luz+MT+Glu组大鼠海马内5-HT含量与对照组比较均减少,差异性明显(P<0.05),MT+Glu组较Glu组和Luz+MT+Glu组5-HT含量升高,差异性明显(P<0.05)。结论MT对谷氨酸钠致痫大鼠痫样发作程度、痫样放电有抑制作用,其机制之一是经由其特异性的膜受体,通过某种机制增强5-HT作用,进而发挥抑痫效应。
Objective To investigate the effect of melatonin (MT) on the level of 5-hydroxytryptamine (5-HT) in the hippocampus of epileptic rats induced by Glutamate (Glu) and to study its mechanism of action in suppressing epilepsy. Methods Forty healthy male Sprague-Dawley rats were randomly divided into four groups (n = 10): normal saline control group (NS group), glutamate-induced epileptic group (Glu group), melatonin + glutamate Sodium group (MT + Glu group); Luzidole + melatonin + glutamate group (Luz + MT + Glu group). The changes of behavior and EEG were observed and recorded. The content of 5-HT in hippocampus was detected by immunohistochemistry. Results The results of behavioral observation and EEG showed that there were no epileptiform seizures and epileptiform discharges in NS group. The epileptic seizures were heavier in Glu group and Luz + MT + Glu group (Ⅲ-Ⅴ grade), EEG showed high frequency of epileptiform epileptiform Wave and TM + Glu group had no or only slight attack (0-Ⅱ grade), and no single epileptiform wave was scattered on EEG or occasionally. Immunohistochemical analysis showed that Glu and Luz + MT + Glu group The content of 5-HT in the hippocampus of rats decreased significantly compared with that in the control group (P <0.05). The content of 5-HT in the Glu group and Luz + MT + Glu group increased significantly <0.05). Conclusions MT can inhibit epileptiform activity and epileptiform discharge in epileptic rats induced by sodium glutamate. One of the mechanisms is that MT can enhance the effect of 5-HT through its specific membrane receptor through some mechanisms Epilepsy.