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目的 :观察解酒保肝口服液对小鼠醉酒实验、血清乙醇浓度和肝、胃组织乙醇脱氢酶活性的影响。方法 :将生理盐水和将葛根、甘草等中药用水煎煮制成解酒保肝口服液灌服于小鼠后 3 0min ,灌服白酒 ,记录小鼠翻正反射消失 (醉酒 )至恢复(醒酒 )所需时间 (min) ,及 2 4h内小鼠的死亡只数 ;另以相同操作连续 6d后眼眶取血并处死动物 ,立即取出肝脏和胃 ,分别用生化比色法测定血清乙醇浓度和肝、胃组织乙醇脱氢酶活性 ;结果 :在醉酒实验中 ,与对照组相比服用解酒保肝口服液组小鼠从饮酒到翻正反射消失 (醉酒 )的时间明显延长 (P <0 .0 1) ,醒酒时间明显缩短 ,且小鼠的死亡率明显降低 (P <0 .0 5) ,血清乙醇含量明显降低 ,肝脏ADH高于对照组 ;结论 :解酒保肝口服液具有解酒作用。
Objective : To observe the effect of Jiejiubaogan Oral Liquid on drunkenness test, serum ethanol concentration, and ethanol dehydrogenase activity in liver and stomach tissues. METHODS: Normal saline and pueraria, licorice and other traditional Chinese medicines were decocted and made into a solution of Jiejiubaogan Oral Liquid for 30 minutes after the mice were fed, and white spirits were fed. The mice were recorded for their righting reflexes (drunken) and recovered (decanters). The required time (min) and the number of mice died within 24 hours; in addition, blood was taken from the eyelids and the animals were sacrificed 6 days after the same operation. The liver and stomach were immediately removed and the serum ethanol concentration and liver were measured by biochemical colorimetry respectively. , Gastric tissue alcohol dehydrogenase activity; Results: Compared with the control group, compared with the control group, the time from drinking alcohol to correcting reflection disappeared (drunk) was significantly longer in the drunkenness test group than in the control group (P<0.00). 1) The sobering time was significantly shortened, and the mortality rate of the mice was significantly lower (P < 0.05), the serum ethanol content was significantly lower, and the liver ADH was higher than the control group. Conclusion: Jiejiubaogan Oral Liquid has an anti-alcoholic effect.