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1997-10 Nishizawa et al发表了第一篇关于人类肝炎相关DNA病毒基因,暂命名为输血传播病毒(transfusion transmitted virus,TTV)的论文,周育森et al在国内首先测定了中国人TTV部分基因克隆序列,迄今国内少数实验室也相继开展了TTV感染的分子流行病学、临床和实验研究。初步研究结果表明,TTV感染同输血或血液制品密切相关,可能是导致肝功长期损害ALT异常又一重要致病因子,为探讨山东地区TTV感染存在和基因变异的情况及TTV在人类肝炎中作用和地位。我们以套式聚合酶链反应(nested-PCR)法,对输血后丙型肝炎和非甲～庚型肝炎患者进行TTV检测,并对部分病例进行序列分析,现将结果报告如下。
1997-10 Nishizawa et al published the first essay on the gene of human hepatitis-related DNA virus, tentatively named as transfusion transmitted virus (TTV). Zhou Yuesun et al first measured the partial sequence of Chinese TTV gene in China So far, a small number of domestic laboratories have also carried out a molecular epidemiology of TTV infection, clinical and experimental studies. Preliminary studies have shown that TTV infection is closely related to blood transfusion or blood products, which may be an important virulence factor that causes long-term liver damage and ALT abnormalities. To investigate the presence and genetic variation of TTV infection in Shandong and the role of TTV in human hepatitis And status. We used nested-PCR to detect TTV in patients with hepatitis C and non-hepatitis A-G after transfusion, and analyzed the sequence of some cases. The results are reported as follows.