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研究树突状细胞（ＤＣ）体外诱导的细胞免疫抑制裸鼠移植瘤生长作用及其机理。方法：联合应用粒／巨噬细胞集落刺激因子ＫＭ－ＣＳＦ）及白介素－４（ＩＬ－４）直接从肝癌患者外周血中培养出ＤＣ，以人肝癌细胞系ＨｅｐＧ２肿瘤细胞的肿瘤抗原粗提物刺激ＤＣ，ＤＣ激活同源的Ｔ淋巴细胞，建立裸鼠人肝癌细胞系ＨｅｐＧ２移植瘤模型，以被激活的Ｔ淋巴细胞治疗裸鼠ＨｅｐＧ２移植瘤并观察治疗效果，检测移植瘤标本肿瘤细胞凋亡情况。结果：ＤＣ诱导的Ｔ细胞免疫能够诱导裸鼠人肝癌细胞系ＨｅｐＧ２移植瘤肿瘤细胞大量凋亡从而抑制裸鼠ＨｅｐＧ２移植瘤生长。结论：经肿瘤抗原激活的ＤＣ作为一新概念上的抗肿瘤疫苗有可能在肿瘤的治疗中发挥重要作用。
To investigate the effect of dendritic cells (DCs) on the growth inhibition of transplanted tumors in nude mice and its mechanism. METHODS: DCs were cultured directly from the peripheral blood of patients with hepatocellular carcinoma using a combination of granulocyte-macrophage colony-stimulating factor (KM-CSF) and interleukin-4 (IL-4), and crude extracts of tumor antigens from human hepatoma cell line HepG2 tumor cells were obtained. Stimulate DCs and DCs to activate homologous T lymphocytes, establish human hepatocellular carcinoma cell line HepG2 xenograft model in nude mice, use activated T lymphocytes to treat HepG2 xenografts in nude mice, observe therapeutic effects, and detect tumor cell apoptosis in xenografts. Happening. RESULTS: DC-induced T cell immunity could induce apoptosis of HepG2 tumor cells in nude mice and inhibit the growth of HepG2 xenografts in nude mice. CONCLUSION: DCs activated by tumor antigens as a new conceptual anti-tumor vaccine may play an important role in the treatment of tumors.