论文部分内容阅读
目的观察不同葡萄糖浓度培养的大鼠胸主动脉平滑肌细胞分泌单核细胞趋化蛋白1(MCP-1)的变化,以及罗格列酮(RGZ)对其分泌的影响。方法用不同浓度的葡萄糖和RGZ单独或联合孵育大鼠胸主动脉平滑肌细胞,用ELISA方法检测培养基中MCP-1的水平,Western Blot方法检测各组所收集细胞胞浆中NF-κBp65和IκBα的表达。结果高葡萄糖浓度培养(11.2,22.4mmol/L)的大鼠胸主动脉平滑肌细胞分泌的MCP-1[分别为(340.87±43.92)pg/ml和(664.87±23.07)pg/ml]明显高于对照组[(132.20±5.81)pg/m1],RGZ抑制了高葡萄糖孵育下大鼠胸主动脉平滑肌细胞MCP-1蛋白表达水平并呈浓度依赖性,RGZ拮抗剂GW9662(10μmol/L)预处理可部分拮抗其作用。MCP-1水平的变化与细胞浆NF-κB、IκB的表达变化相伴随。结论高糖可以诱导大鼠胸主动脉平滑肌细胞分泌MCP-1,并呈现浓度依赖性;RGZ可抑制高糖诱导的大鼠胸主动脉平滑肌细胞分泌MCP-1水平,上述作用在一定浓度范围内呈现剂量依赖性。高糖诱导平滑肌细胞分泌MCP-1很可能是通过NF-κB通路来调控的。
Objective To observe the changes of monocyte chemoattractant protein-1 (MCP-1) secreted by rat aortic smooth muscle cells cultured in different glucose concentrations and the effect of rosiglitazone (RGZ) on its secretion. Methods Rat thoracic aortic smooth muscle cells were incubated singly or in combination with different concentrations of glucose and RGZ. The levels of MCP-1 in culture medium were detected by ELISA. The levels of NF-κBp65 and IκBα in the cytoplasm were detected by Western Blot. expression. Results MCP-1 secreted by rat thoracic aortic smooth muscle cells (340.87 ± 43.92 pg / ml and (664.87 ± 23.07) pg / ml, respectively) was significantly higher in rats with high glucose (11.2,22.4 mmol / L) RGZ could inhibit the expression of MCP-1 protein in rat aorta smooth muscle cells under high glucose concentration in a concentration-dependent manner and RGZ antagonist GW9662 (10μmol / L) pretreatment Can partially antagonize its role. The change of MCP-1 level was accompanied by the change of expression of NF-κB and IκB in cytoplasm. Conclusion High glucose can induce MCP-1 secretion in rat aortic smooth muscle cells in a concentration-dependent manner. RGZ can inhibit MCP-1 secretion induced by high glucose in rat aortic smooth muscle cells. Dose-dependent. High glucose-induced smooth muscle cell secretion of MCP-1 is likely to be regulated by the NF-κB pathway.