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2002年10-11月,应用聚丙烯酰胺凝胶水平电泳,对我国新疆丁群体和从捷克引进的丁鱼岁群体各30尾个体的ADH、IDH、LDH、SDH、SOD、EST、MDH、ME、6PGDH、G3PDH等10种同工酶和1种肌蛋白进行分析。结果发现,我国丁鱼岁群体在EST-2、SOD、ADH和IDH等4种同工酶存在多态,捷克群体在EST-1、ADH和IDH等3种同工酶存在多态。我国新疆丁群体多态位点比例为28.57%,比捷克丁群体高(21.43%)。平均杂合度分别为0.061 9,也比捷克丁群体高(0.035 7)。捷克丁群体存在一定的杂合子缺失现象。因此,从遗传保护角度,应防止引入我国的捷克丁鱼岁群体进入新疆额尔齐斯河流域进行人工养殖,以免对我国丁天然资源造成污染。
From October to November in 2002, ADH, IDH, LDH, SDH, SOD, EST, MDH, LDH, ADH, LDH and SOD of 30 individuals of 30 individuals of Ding Ding population in Xinjiang and 30 populations of Dingyu imported from Czech were detected by polyacrylamide gel electrophoresis. ME, 6PGDH, G3PDH and other 10 kinds of isozymes and 1 kinds of muscle protein analysis. The results showed that there were polymorphisms in four isozymes of EST-2, SOD, ADH and IDH in the population of Chinese anchovy. Polymorphism was found in EST-1, ADH and IDH in the Czech population. The proportion of polymorphic loci in Dingjian population in China was 28.57%, which was 21.43% higher than that in the Czech Dingjuan population. The average heterozygosity was 0.061 9, respectively, also higher than the Czech Ding population (0.035 7). There is a certain loss of heterozygotes in the Czech population. Therefore, from the perspective of genetic protection, it is necessary to prevent the aged population of Czech cichlids introduced into our country from entering the Irtysh River basin in Xinjiang for artificial breeding in order to avoid the pollution of the natural resources of Dingjian in our country.