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目的寻找快速、灵敏、特异的检测伤寒抗原的方法。方法制备提取纯化免疫抗伤寒沙门菌体O901株（IgM）；鞭毛H901株（IgG）多克隆抗体，经Westernblot分析发现能特异地识别“O”66．07KD；“H”54．09KD抗原条带，正常对照均无抗原条带显示。利用该抗体特异性，运用免疫酶斑点法（DI－PAP）对标准株．伤寒“O”、“H”株抗原及血培养确诊为伤寒病患者血清50例作为抗原进行检测。结果抗原点染色明显出现棕黄色斑点。50例中O抗原48例阳性；H抗原46例为阳性，检出率分别为96％与92％，正常对照均为阴性，P＜0．01。结论两种特异性抗体具有较好敏感性、可靠性，可作为伤寒抗原的早期诊断指标。
Objective To find a rapid, sensitive and specific method for the detection of typhoid antigens. Methods The polyclonal antibody against the purified anti-Salmonella typhimurium O901 strain (IgM) and flagella H901 strain (IgG) was prepared and purified by Western blotting. The results showed that the antibody could specifically recognize “O” 66.07KD. The “H” 54.09KD antigen band No antigen band showed in normal control. Using this antibody specificity, the use of immunoenzymatic dot blot (DI-PAP) against the standard strain. Typhoid “O”, “H” strain antigen and blood culture diagnosed as 50 cases of typhoid patients serum as antigen for testing. The results of the stain spots of brown spots appear obvious. Forty-eight patients were positive for O-antigen in 50 cases, and 46 cases were positive for H antigen. The detection rates were 96% and 92%, respectively. The positive control was negative (P <0.01). Conclusion The two specific antibodies have good sensitivity and reliability and can be used as an early diagnostic indicator of typhoid antigen.