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为了了解早代PPO活性的分布情况,以多巴(L-DOPA/MOPS)为底物,采用全麦粉过滤法测定了小麦组合(扬麦158×淮麦18)早代(F2和F2∶3家系群体)的PPO活性。结果表明,F2和F2∶3家系群体的PPO活性分布均呈明显的偏态分布,且F2和F2∶3家系群体的PPO活性极显著相关,相关系数为0.727。用单个分离世代群体遗传模型分析方法对该组合的F2群体、F2∶3家系群体的PPO活性进行遗传分析,结果表明,该组合的PPO活性主要受两对主基因控制,且主基因表现为加性-显性效应。F2群体控制PPO活性的两对主基因遗传率为73.92%,F2∶3家系群体中两对主基因遗传率为88.44%。
In order to understand the distribution of PPO activity in early generation, the combination of wheat (Yangmai 158 × Huaimai 18) and F2 (F2:3) was determined by whole wheat flour filtration method using L-DOPA / MOPS as substrate. Pedigree population) PPO activity. The results showed that there was a significant skewed distribution of PPO activity in F2 and F2: 3 pedigrees, and PPO activity in F2 and F2: 3 pedigrees was significantly correlated with a correlation coefficient of 0.727. Genetic analysis of PPO activity in the F2 population and F2: 3 population using a single segregation population genetic model showed that the PPO activity of the combination was mainly controlled by two pairs of major genes and the major genes showed additive Sex - Dominant effect. The heritability of two pairs of major genes of F2 population controlling PPO activity was 73.92%, and the heritability of two major genes in F2: 3 population was 88.44%.