论文部分内容阅读
目的：探讨噪声对豚鼠耳蜗核复合体(CNC)内一氧化氮合酶(NOS)阳性神经元数目和面积的影响。方法：采用依赖还原型辅酶Ⅱ的尼克酰胺腺嘌呤二核苷酸磷酸黄递酶(NADPH-d)组织化学方法，观察正常组和噪声损伤组(噪声损伤后1 d，1周，4周)CNC内NOS阳性神经元数目和面积的变化。结果：与正常组相比，噪声损伤后1d至4周，CNC内NOS阳性神经元数目逐渐增多；噪声损伤后1d NOS阳性神经元面积急剧缩小，噪声损伤后1～4周NOS阳性神经元面积逐渐升高并超过正常水平。与正常组比较，噪声损伤后1 d，1周，4周组，NOS阳性神经元面积和数目差异有显著性(P＜0.05)，噪声损伤后1 d，l周，4周3组之间差异亦有显著性(P＜0.05)。结论：在噪声损伤后，NO释放增多，对听觉核团有复杂的调节作用。“,”Aim: To study the expression of nitric oxide synthetase (NOS) positive neurons(number and area) in cochlearnucleus complex (CNC) of guinea pigs with temporary noise-induced deafness. Methods: Nicotinamidc adenine dinucleotidephosphate-diaphorase (NADPH-d) histochemistry method was adopted to study the expression of NOS positive neurons( number andarea) in CNC in normal group and 3 noise-induced deafness groups (alive 1 d, 1 week and 4 weeks after exposed to the strongnoise). Fesults: Compared with normal group, the number of NOS positive neurons in CNC increased during the period from ldto 4weeks after the noise-exposure. The area of NOS positive neurons in CNC first decreased promptly ( ld after the noise-expo-sure) and almost continuously increased afterwards. There were significant differences not only between the noise-induced deafnessgroups and normal group(P＜0.05) but between the 3 noise-induced deafness groups(P＜0.05). Cpmclusion: It suggests thatthe release of NO increases after noise-exposure. NO has complex regulative effects on CNC.