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In this study,the event-specific real-time fluorescence quantitative PCR method established by Sichuan Academy of Agricultural Sciences was employed to detect the content of flanking fragment( specific fragment) in samples containing 2% genetically modified maize event NK603. The uncertainty of detection results was evaluated based on various uncertainty sources,such as PCR amplification system,data analysis and micropipette. The results showed that A-type uncertainty( uA),B-type uncertainty( uB),combined standard uncertainty( uC) and expanded uncertainty( U95) were0.000 8,0.001,0.001 and0.002,respectively; the final detection result was 1. 9% ± 0. 002. Thus,the main uncertainty in detecting flanking fragment of genetically modified maize event NK603 with realtime fluorescence quantitative PCR method was derived from the random effect in the experimental process
In this study, the event-specific real-time fluorescence quantitative PCR method established by Sichuan Academy of Agricultural Sciences was employed to detect the content of flanking fragment (specific fragment) in samples containing 2% genetically modified maize event NK603. The uncertainty of detection results was evaluated based on various uncertainty sources, such as PCR amplification system, data analysis and micropipette. The results showed that A-type uncertainty (uA), B-type uncertainty (uB), combined standard uncertainty (uC) and expanded uncertainty U95) were0.000 8,0.001,0.001 and 0.002, respectively; the final detection result was 1. 9% ± 0.002. Thus, the main uncertainty in detecting flanking fragment of genetically modified maize event NK603 with realtime fluorescence quantitative PCR method was derived from the random effect in the experimental process