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[Objective] To research the effects of medicated serum of HERBA POLYGONI PERFOLIATI on HBsAg and HBeAg secretion in2215 cells. [Methods]Destruction rate of medicated serum of HERBA POLYGONI PERFOLIATI to 2215 cells and HL-7702 were detected by MTT method; HBsAg and HBeAg secretion in cell supernatant was detected by ELISA method when four different concentrations of medicated serums were applied in 2215 cells for 72 h. [Results]20% and 40% medicated serums showed inhibitory effects on HBsAg and HBeAg secretion,showing certain dose-effect relationship. At the same time,medicated serum of HERBA POLYGONI PERFOLIATI had less significant inhibitory effects on HL-7702 than that on 2215 cells. [Conclusions] Medicated serum of HERBA POLYGONI PERFOLIATI had certain toxic and anti-HBV effects in vitro.
[Objective] To research the effects of medicated serum of HERBA POLYGONI PERFOLIATI on HBsAg and HBeAg secretion in 2215 cells. [Methods] Destruction rate of medicated serum of HERBA POLYGONI PERFOLIATI to 2215 cells and HL-7702 were detected by MTT method; HBsAg and HBeAg secretion in cell supernatant was detected by ELISA method when four different concentrations of medicated serums were applied in 2215 cells for 72 h. [Results] 20% and 40% medicated serums have inhibitory effects on HBsAg and HBeAg secretion, showing certain dose-effect relationship At the same time, medicated serum of HERBA POLYGONI PERFOLIATI had less significant inhibitory effects on HL-7702 than that on 2215 cells. [Conclusions] Medicated serum of HERBA POLYGONI PERFOLIATI had certain toxic and anti-HBV effects in vitro.