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为了研究心肌纤维化与高血压性左室肥厚引起的心功能障碍的关系,我们采用苦味酸天狼猩红染色偏振光法显示I型及Ⅲ型胶原纤维,效果良好,介绍如下。1材料和方法1.1材料和染液取小鼠的心肌组织,经10%福尔马林固定,石蜡包埋,切片厚3~6μm。染液配方:①苦味酸天狼猩红染?
In order to study the relationship between myocardial fibrosis and hypertensive left ventricular hypertrophy caused by cardiac dysfunction, we use picric acid Sirius red staining polarized light method shows type I and type III collagen fibers with good results, described below. 1 Materials and methods 1.1 Materials and dye solution Myocardial tissue from mice, fixed by 10% formalin, embedded in paraffin, section thickness 3 ~ 6μm. Dye formula: picric acid Sirius red dye?