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目的研究衢州地区708例宫颈病变女性感染各亚型人乳头状瘤病毒(HPV)的概率,探讨单一感染、双重感染、多重复合感染等感染机会分布情况。方法采用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术检测宫颈脱落细胞HPV亚型,按不同分组统计分析708例宫颈病变女性感染不同亚型人乳头状瘤病毒(HPV)的机会。结果 708例感染标本检出各亚型阳性点数为1 001次,单一亚型感染占71.13%,双重感染占24.18%以及多重复合占4.70%,其中不同亚型在各感染机会中占所有阳性点位的比例为:高危型分别占49.05%、18.88%、4.00%,低危型分别占22.08%、5.29%、0.70%。高危型和低危型HPV感染机会的差异具有统计学意义。各种感染机会在不同年龄段分布比例不同,31岁~40岁年龄段单一亚型感染比例最高(47.60%),比例差异均有统计学意义(χ2=256.29,P<0.005)。结论 HPV感染机会具有明显的亚型和年龄分布异质性,混合感染比例不容忽视,提示使用HPV预防性疫苗应同时考虑各亚型的感染机会和年龄因素。
Objective To investigate the incidence of HPV subtypes in 708 cases of cervical lesions in Quzhou district and to explore the distribution of infection opportunities such as single infection, double infection and multiple compound infection. Methods The HPV subtype of exfoliated cells of cervical exfoliated cells was detected by DNA microarray combined with PCR in vitro amplification and reverse dot blot hybridization. The data of 708 HPV subtypes in women with cervical lesions were statistically analyzed by different groups . Results Among 708 samples, 1 001 positive spots were detected in each subtypes, 71.13% in single subtypes, 24.18% in double infection and 4.70% in multiple subtypes. Among them, different subtypes accounted for all positive points in each infection The proportion of high-risk persons was 49.05%, 18.88% and 4.00% respectively, while low-risk persons accounted for 22.08%, 5.29% and 0.70% respectively. The differences in the chances of high-risk and low-risk HPV infection were statistically significant. The infection rate of various infections varied in different age groups, with the highest proportion of single subtype infection (47.60%) between 31 years old and 40 years old. There were significant differences in the proportions (χ2 = 256.29, P <0.005). Conclusion HPV infection has obvious subtypes and age distribution heterogeneity. The proportion of mixed infections should not be neglected. It suggests that the use of HPV vaccines should take into account both infection chance and age factors.