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通过施用茶氨酸来减轻低钾胁迫对烟草幼苗的毒害,并从抗氧化系统、钾离子含量及相关基因来探讨其作用机理。结果表明,在低钾胁迫下,0.1和0.2 mmol·L-1茶氨酸均能促进烟苗根长、鲜重及叶绿素含量的显著增加;外源茶氨酸预处理不仅能显著提高低钾胁迫下烟草幼苗脯氨酸、谷胱甘肽(GSH)含量,还能增强烟草幼苗抗坏血酸还原酶(APX)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,降低过氧化氢(H2O2)和丙二醛(MDA)含量。此外,茶氨酸还能促进烟草幼苗对钾离子的吸收。半定量PCR结果表明,茶氨酸预处理可以诱导低钾胁迫下烟草幼苗Nt LKS1、Nt KC和Nt KT1上调。综上,茶氨酸缓解烟草幼苗低钾胁迫是通过激发植物体抗氧化系统、促进钾离子的吸收及相关基因上调的综合结果。
The theanine was used to reduce the toxicity of low potassium stress to tobacco seedlings and the mechanism of action was discussed based on the antioxidant system, potassium ion content and related genes. The results showed that under low potassium stress, 0.1 and 0.2 mmol·L-1 theanine all promoted the root length, fresh weight and chlorophyll content of tobacco seedlings significantly increased. Exogenous theanine pretreatment could not only significantly increase the content of potassium Proline and glutathione (GSH) contents in tobacco seedlings under stress also increased the activity of ascorbate reductase (APX), peroxidase (POD) and catalase (CAT) in tobacco seedlings and decreased the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) content. In addition, theanine can also promote tobacco seedling potassium absorption. Semi-quantitative PCR results showed that the theanine pretreatment can induce low potassium stress Nt LKS1, Nt KC and Nt KT1 up-regulation of tobacco seedlings. In summary, the theanine to alleviate low potassium stress in tobacco seedlings is through the antioxidant system of plants to promote and promote the absorption of potassium ions and related genes up the overall results.