论文部分内容阅读
目的观察常通口服液对术后粘连大鼠血液中肿瘤坏死因子α(TN F-α)、转化生长因子β1(TG F-β1)、白细胞介素-1β、4、6、10(IL-1β、IL-4、IL-6、IL-10)水平的影响。方法选用SD 雄性大鼠,随机分为6 组:正常对照组、模型对照组、四磨汤组及常通口服液低、中、高剂量组。除正常对照组外,其余大鼠均按Ellis法制备成肠粘连模型。正常及模型对照组予以蒸馏水灌胃(10 m l/kg);四磨汤组以10 m l/kg 灌胃给药;常通口服液低、中、高剂量组分别按4.3、8.6 、17.2 g/kg 灌胃给药。各组于术后第7 天取血,酶联免疫吸附法测定细胞因子水平,同时进行粘连程度肉眼分级。结果常通口服液能显著减轻模型大鼠的肠粘连程度,降低术后肠粘连大鼠血清中致炎细胞因子TN F-α、IL-1β、TG F-β1、IL-6 水平,但对抗炎细胞因子IL-4、IL-10 水平无影响。结论本实验初步筛选出有意义的术后粘连疗效评价指标,为术后粘连临床疗效评价和新药研发建立客观、准确、可靠的临床疗效评价指标提供了实验依据。
Objective To observe the effect of Changtong Oral Liquid on tumor necrosis factor α (TNF-α), transforming growth factor β1 (TG F-β1), interleukin-1β, 4,6,10 (IL- 1β, IL-4, IL-6, IL-10) levels. Methods SD male rats were randomly divided into 6 groups: normal control group, model control group, Sihuangtang group and Changtong oral liquid low, medium and high dose groups. Except normal control group, the other rats were made into intestinal adhesion model according to Ellis method. Normal and model control groups were given distilled water (10 ml / kg); Sihuangtang group was orally administrated at 10 ml / kg; the low, medium and high dose of Changtong Oral Liquid was administered at 4.3, 8.6, 17.2 g / kg intragastric administration. Blood was collected on the 7th postoperative day in each group, and cytokine levels were measured by enzyme-linked immunosorbent assay. At the same time, the degree of adhesion was graded by naked eye. Results Changtong Oral Liquid can significantly reduce the degree of intestinal adhesion and decrease the serum levels of inflammatory cytokines TN F-α, IL-1β, TG F-β1 and IL-6 in rats with intestinal adhesion Anti-inflammatory cytokines IL-4, IL-10 levels had no effect. Conclusions This experiment screened out significant indexes of postoperative adhesion evaluation, which provided the experimental evidences for the objective, accurate and reliable clinical efficacy evaluation of postoperative adhesion evaluation and new drug development.