论文部分内容阅读
目的研究骨形态发生蛋白(BMP)对兔骨髓间充质干细胞(MSCs)的定向诱导作用,探讨MSCs的成软骨能力。方法分离并培养兔MSCs,BMP诱导MSCs向软骨细胞分化,观察其形态学改变及碱性磷酸酶(ALP)活性。将诱导后的MSCs接种PGA无纺网体外培养2周,将MSCsPGA无纺网复合体植入裸鼠背部皮下,2个月后取材,进行组织学观察。结果经BMP诱导后,MSCs的形态由长梭形向多角形和三角形转化,群体倍增时间约为3.0d。诱导5、10d后,ALP活性为0.282±0.015,0.502±0.012,明显高于对照组0.265±0.010,0.315±0.021(P<0.01),ALP染色阳性。MSCsPGA无纺网复合体植入裸鼠后,组织学可见软骨陷窝,陷窝内可见核蓝染的成软骨细胞,证实MSCs具有体内成软骨能力。结论经BMP诱导的MSCs向软骨细胞分化和增殖,在裸鼠体内可形成软骨组织。
OBJECTIVE: To investigate the osteoclastogenic ability of bone marrow mesenchymal stem cells (MSCs) induced by bone morphogenetic protein (BMP). Methods Rabbit MSCs were isolated and cultured. BMPs induced differentiation of MSCs into chondrocytes. Morphological changes and alkaline phosphatase (ALP) activity were observed. The induced MSCs were seeded on PGA nonwoven mesh and cultured in vitro for 2 weeks. The MSCsPGA non-woven mesh composite was subcutaneously implanted into the back of nude mice. After 2 months, the cells were harvested for histological observation. Results After BMP induction, the morphology of MSCs transformed from long fusiform to polygonal and triangular, and the population doubling time was about 3.0 d. ALP activity was 0.282 ± 0.015 and 0.502 ± 0.012 after induction for 5 and 10 days respectively, which was significantly higher than that of the control group (0.265 ± 0.010,0.315 ± 0.021, P <0.01). ALP staining was positive. MSCsPGA non-woven mesh composite implanted in nude mice, the cartilage lacuna can be seen histologically, the nest can be seen in the nuclear blue dye into chondrocytes, confirmed that MSCs have the capacity of cartilage in vivo. Conclusion BMP-induced MSCs differentiate into chondrocytes and proliferate, forming cartilage in nude mice.