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为了探讨养殖水域中分子氨对水生生物的毒性效应,在前期毒性试验的基础上设置3个试验组(分子氨质量浓度分别为0.071,0.143,0.284mg/L)、1个对照组对黄河鲤鱼(Cyprinus carpio haematopterus Temmink etSchlegel)进行毒性试验,在试验的7,14,28,35d取样检测,测定试验鱼血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力和丙二醛(MDA)质量浓度.结果表明:随着分子氨质量浓度的增加,黄河鲤鱼血清中SOD酶活力呈先升后降的趋势,质量浓度为0.28mg/L时,SOD活力低于对照组,GSH-Px的活力和MDA质量浓度呈现上升的趋势,方差分析表明分子氨极显著地提高血清MDA质量浓度(p<0.01),二者存在剂量—效应关系;随着分子氨暴露时间的延长,SOD活力呈下降趋势,MDA质量浓度呈上升趋势,方差分析差异不显著(p>0.05).在试验质量浓度范围内分子氨可降低鲤鱼抗氧化能力,且MDA可作为一种有效的生物标志物评价分子氨的毒性.
In order to investigate the toxic effect of molecular ammonia in aquaculture waters on aquatic organisms, three experimental groups (molecular mass concentrations of 0.071,0.143,0.284 mg / L, respectively) were set up on the basis of previous toxicity test. One control group (Cyprinus carpio haematopterus Temmink et Schlegel) were used to test the toxicity of SOD and GSH-Px in fish at 7, 14, 28 and 35 days. The results showed that with the increase of molecular mass concentration of ammonia, the activity of SOD in serum increased first and then decreased. At the concentration of 0.28 mg / L, SOD activity The activity of GSH-Px and the concentration of MDA showed a rising trend lower than the control group. The analysis of variance showed that the molecular weight of ammonia significantly increased the concentration of serum MDA (p <0.01), both of which had dose-effect relationship. With the increase of molecular ammonia In the range of experimental concentration, molecular ammonia reduced the antioxidant capacity of carp, and MDA could be used as a kind of anti-oxidant Effective biomarker Evaluation of molecular toxicity of ammonia.