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目的评价刀额新对虾主要变应原36kD和68kD蛋白在临床检测虾过敏症特异性IgE方面的应用。方法以纯化的刀额新对虾主要变应原36kD和68kD蛋白为抗原包被酶标反应板,采用间接ELISA法检测1301份人血清样品中的特异性IgE,对其中血清特异性IgE阳性者进行虾类食物过敏反应的回顾性调查。结果1301份血清中,特异性IgE阳性的有127份,阳性率为9.76%(127/1301)。回顾性调查表明,特异性IgE阳性个体均有虾类食物过敏史,与血清学检测结果相一致。结论刀额新对虾主要变应原36kD和68kD蛋白针对血清特异性IgE的检测具有较高的特异性和敏感性,适用于研制临床检测虾过敏症的ELISA试剂。
Objective To evaluate the application of 36 kD and 68 kD proteins of major shrimp neoplasms in the clinical detection of shrimp allergy-specific IgE. Methods The purified 36kD and 68kD protein of major shrimp neoplasms were coated with enzyme-labeled reaction plate, and the specific IgE in 1301 human serum samples were detected by indirect ELISA. Serum-specific IgE positive samples were detected Retrospective investigation of allergic reactions in shrimp food. Results Among the 1301 serum samples, 127 were positive for specific IgE and the positive rate was 9.76% (127/1301). Retrospective survey showed that specific IgE positive individuals have history of shrimp food allergy, consistent with serological test results. Conclusion The 36 kD and 68 kD proteins of major allergen of Penaeus vannamei have high specificity and sensitivity for the detection of serum specific IgE, and are suitable for the development of ELISA reagents for clinical detection of shrimp allergy.