论文部分内容阅读
Wolbachia是一类广泛存在于节肢动物体内,可以对寄主的生殖力及生殖行为产生影响的共生菌。用抗生素可以有效除去寄主体内的Wolbachia。本实验通过喂食浓度分别为1,5和10mg盐酸四环素/mL蔗糖水,结合PCR检测丽蚜小蜂Encarsia formosa体内Wolbachia的去除效果;解剖观察丽蚜小蜂F0代及F1,F2和F3代怀卵量和卵巢管数量,评价Wolbachia对丽蚜小蜂生殖的影响。结果显示:抗生素处理去除Wolbachia后F0代蜂的卵巢管数量与未处理蜂之间无显著差异(P=0.12),但F1,F2和F3代蜂的卵巢管均为6条,显著少于F0代蜂的卵巢管数量(P<0.001)。抗生素处理去除Wolbachia后的F0代蜂怀卵量与未处理蜂相比显著下降,但显著高于经抗生素处理去除Wolbachia后的F1,F2和F3代蜂怀卵量(P<0.001),后代(F1,F2,F3)蜂之间怀卵量无显著差异(P=0.59)。去除Wolbachia后,丽蚜小蜂可以产生雄性后代,但未见交配行为,且雌蜂可不经交配而产生雌性后代。结果说明,Wolbachia不仅直接影响丽蚜小蜂的怀卵量,而且还可以通过影响丽蚜小蜂卵巢管的发育影响丽蚜小蜂的怀卵量;然而,去除Wolbachia不改变雌蜂的孤雌生殖方式。
Wolbachia is a group of commensal bacteria that are widespread in arthropods and have an effect on the reproductive and reproductive behavior of the host. Antibiotics can effectively remove Wolbachia from the host. In this experiment, Wolbachia removal efficiencies of 1,5 and 10 mg tetracycline hydrochloride / mL sucrose hydrochloride were tested respectively by PCR, and F0 generation and F1, F2 and F3 offspring The amount of ovary and the amount of ovarian tube to evaluate the effects of Wolbachia on the reproduction of the aphid larvae. The results showed that there was no significant difference (P = 0.12) between the number of ovarian duct and the untreated bee in F0 wasp after antibiotic treatment, but the number of ovary in F1, F2 and F3 was significantly lower than that of F0 The number of ovarian tubes on behalf of the bee (P <0.001). The fecundity of F0 offspring after antibiotic treatment was significantly lower than that of the untreated bee, but significantly higher than that of the F1, F2 and F3 offspring after antibiotic treatment (P <0.001) F1, F2, F3) had no significant difference (P = 0.59). After removal of Wolbachia, aphid wasps can produce male offspring, but no mate behavior, and females can produce female offspring without mating. The results showed that Wolbachia not only directly affects the fecundity of the queen bee, but also affects the fecundity of the queen bee by affecting the ovarian development of the queen bee; however, removal of Wolbachia does not alter parthenogenesis Reproductive methods.