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目的探讨环氧合酶2(COX-2)抑制剂对胰腺癌细胞增殖、侵袭、迁移能力和对COX-2、基质金属蛋白酶(MMP)-14蛋白表达的影响以及可能的抗胰腺癌机制。方法不同浓度的COX-2抑制剂塞来昔布(20、60、100μmol/L)处理胰腺癌细胞后,用MTT比色法检测细胞的增殖能力;用Transwell侵袭实验和划痕实验检测细胞的侵袭能力和迁移能力;ELISA检测MMP-14和COX-2的蛋白表达情况。结果 MTT增殖实验、Transwell侵袭实验、划痕实验分别提示,COX-2抑制剂作用后胰腺癌细胞的增殖、侵袭、迁移能力均以浓度梯度形式下降(P<0.05);ELISA结果显示,胰腺癌细胞中COX-2和MMP-14的蛋白表达水平相应降低(P<0.05),两者表达具有显著正相关性(r=0.873,P<0.05)。结论 COX-2抑制剂可能通过抑制COX-2表达下调MMP-14表达,进而以浓度梯度形式减弱胰腺癌细胞的增殖、侵袭、迁移能力,起到抗胰腺癌作用。
Objective To investigate the effects of cyclooxygenase 2 (COX-2) inhibitor on the proliferation, invasion, migration and the expression of COX-2 and MMP-14 in pancreatic cancer cells and the possible mechanism of anti-pancreatic cancer. Methods After the cells were treated with different concentrations of celecoxib (20, 60 and 100 μmol / L), the proliferation of pancreatic cancer cells was detected by MTT assay. The cell proliferation was evaluated by Transwell invasion assay and scratch assay Invasion ability and migration ability; ELISA detection of MMP-14 and COX-2 protein expression. Results MTT proliferation assay, Transwell invasion assay and scratch assay suggested that the proliferation, invasion and migration of pancreatic cancer cells decreased with the concentration gradient after COX-2 inhibitor treatment (P <0.05). The results of ELISA showed that pancreatic cancer The protein expression of COX-2 and MMP-14 in the cells decreased accordingly (P <0.05), and there was a significant positive correlation between the expression of COX-2 and MMP-14 (r = 0.873, P <0.05). Conclusions COX-2 inhibitors may down-regulate the expression of MMP-14 by inhibiting the expression of COX-2, and then attenuate the proliferation, invasion and migration of pancreatic cancer cells in a concentration gradient manner and thus play a role in anti-pancreatic cancer.