本研究采用基因工程技术,构建一种重组霍乱毒素B亚单位（rCT-B）的高效表达/分泌系统。 将CT-B结构基因ctxB与修饰的ompA信号序列融合,然后根据鼠伤寒杆菌阿拉伯糖操纵子的调节信号克隆入M13噬菌体并感染大肠杆菌。将此大肠杆菌接种于含0.2％阿位伯糖的LB肉汤中培养,检测培养物中rCT-B含量并通过S-SepharoseFF柱进行纯化。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、等电聚焦、质谱分析等方法对rCT-B的特性进行研究。 In this study, a gene engineering technique was used to construct an efficient expression / secretion system of recombinant cholera toxin B subunit (rCT-B). The CT-B structural gene ctxB was fused to the modified ompA signal sequence and then cloned into M13 phage and infected with E. coli based on the regulatory signal of the Salmonella typhimurium arabinose operon. The E. coli was inoculated in LB broth containing 0.2% of azobarose, the content of rCT-B in the culture was detected and purified by an S-Sepharose FF column. The characteristics of rCT-B were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing and mass spectrometry.