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已知神经肽在机体消化活动及其调控中具有重要作用,但有关人类肠道肽能神经发生的研究仍然缺乏。本实验采用PAP法,对47例人工流产的新鲜人胎肠神经系中VIP、SP、CGRP、NPY、ENK5种神经肽进行了观察,并对其神经纤维密度进行了显微分光光度计测定。 47例月经龄8～40周的胎儿按月分为8组,每组3～11例,取大肠及小肠各段入2.5％ Zambonis液固定,20％蔗糖过夜,恒冷箱切片机切片(厚25μm),常规PAP漂浮法显示神经肽,并有严格的方法对照。 11周时肌间丛和粘膜下丛中可见NPY阳性神经纤维;第6月,肠绒毛内可见神经纤维;7～9月
Neuropeptides are known to play an important role in the digestive activity and regulation of the body, but studies on the human gut peptide neurogenesis are still lacking. In this experiment, we used the PAP method to observe the neuropeptides of VIP, SP, CGRP, NPY and ENK in 47 cases of abortion human embryonic nervous system. The nerve fiber density was measured by spectrophotometer. Forty-seven fetuses with 8 ~ 40 weeks of menstruation were divided into 8 groups on a monthly basis, ranging from 3 to 11 cases. The sections of the large intestine and small intestine were fixed with 2.5% Zambonis solution and 20% sucrose overnight. 25 [mu] m), conventional PAP float method shows neuropeptides and has rigorous method controls. At week 11, NPY positive nerve fibers were seen in the muscular plexus and submucosal plexus. In the first 6 months, nerve fibers were seen in the villi of the intestinal villi. From July to September