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在抗旱鉴定大棚内同时实现水、旱2种处理,测定了水稻珍汕97B和旱稻IRAT109杂交F9代重组自交系群体(195个株系)的冠层温度(canopytemperature,CT)、叶水势(leafwaterpotential,LWP)和结实率(spikeletfertility,SF).相关分析结果表明,干旱条件下,冠层温度与结实率呈极显著负相关(?0.2867);叶水势与结实率呈显著正相关(0.1696);冠层温度与叶水势呈极显著负相关(?0.2740),说明在干旱条件下保持较低冠层温度及较高叶水势的材料抗旱性较强.利用QTLMapper共检测到44个主效QTL和45对显著的互作位点与冠层温度、叶水势和结实率的表达有关.在水、旱条件下,主效QTL对CT,LWP和SF的联合贡献率分别为87.85%,15.06%,79.46%和72.61%,87.68%,33.29%;互作位点对CT,LWP和SF的联合贡献率分别为55.69%,47.16%,48.15%和53.44%,57.94%,54.62%.与已有的抗旱QTL定位结果比较,发现有19个主效QTL与已定位的抗旱QTL位于相同的或紧密相连的染色体区段.
Water and drought treatments were simultaneously implemented in drought-resistant identification greenhouse. Canopy temperature (CT), leaf water potential (CT), and leaf water potential were measured in 195 inbred lines of F9 hybrids of Zhen- leaf water potential, LWP and spikeletfertility (SF). Correlation analysis showed that there was a significant negative correlation between canopy temperature and seed setting rate ( 0.2867) under drought conditions; leaf water potential was positively correlated with seed setting rate (0.1696) (0.2740), indicating that the drought resistance of the material with the lower canopy temperature and the higher leaf water potential was stronger under drought conditions.A total of 44 major-effect QTLs were detected using QTLMapper And 45 pairs of significant interaction sites were related to the expression of canopy temperature, leaf water potential and seed setting rate.The combined effect of major QTLs on CT, LWP and SF under water and drought conditions was 87.85% and 15.06% , 79.46% and 72.61%, 87.68% and 33.29% respectively. The combined contribution rates of the interaction sites to CT, LWP and SF were 55.69%, 47.16%, 48.15% and 53.44%, 57.94% and 54.62% Of drought-resistant QTLs were compared and found that 19 major QTLs located in the same or closely linked with the mapped QTLs Body-color section.