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目的: 观察恒河猴接种Aβ42肽疫苗后的特异性抗体的产生。方法: 将 5只雄性恒河猴分别在 0、2、6、10、14、18、22wk肌内注射Aβ42肽疫苗; 用ELISA法检测恒河猴血清抗Aβ42抗体水平及IgG亚类; 用Westernblot检测血清抗Aβ42抗体的特异性; 免疫组化染色法观察抗血清对Tg2576转基因小鼠脑组织中Aβ斑的识别。结果: 疫苗接种后第 8周, 恒河猴血清中出现明显的抗Aβ42抗体, 抗体水平随着接种次数的增加而升高, 第 24周达 1∶4 320, 以后抗体水平开始下降。产生的抗Aβ42抗体以IgG1和IgG2为主 (IgG2 /IgG1 >1)。血清抗Aβ42抗体具有高度特异性, 可识别Tg2576转基因小鼠脑组织中的Aβ斑。结论: Aβ42肽疫苗可有效地诱导恒河猴产生特异性体液免疫应答。
OBJECTIVE: To observe the production of specific antibodies after rhesus monkeys inoculated with Aβ42 peptide vaccine. Methods: Five male Rhesus monkeys were intramuscularly injected with Aβ42 peptide vaccine at 0, 2, 6, 10, 14, 18, and 22 weeks respectively. Serum anti-Aβ42 antibody levels and IgG subclasses were determined by ELISA. Western blot The specificity of serum anti-Aβ42 antibody was detected. Immunohistochemical staining was used to observe the recognition of Aβ plaques in Tg2576 transgenic mice brain by antiserum. Results: At the 8th week after vaccination, the anti-Aβ42 antibody appeared in the serum of rhesus monkeys. The level of antibody increased with the increase of inoculation frequency. At the 24th week, the antibody level began to decrease. The resulting anti-Aβ42 antibodies are predominantly IgG1 and IgG2 (IgG2 / IgG1> 1). Serum anti-Aβ42 antibodies are highly specific and recognize Aβ plaques in brain tissue of Tg2576 transgenic mice. Conclusion: Aβ42 peptide vaccine can effectively induce specific humoral immune response in rhesus monkeys.