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目的：探讨谷胱甘肽过氧化物酶1（GPx-1）基因C594T多态性与急性脑梗死的关系。方法用聚合酶链反应-限制性片段长度多态性分析法（ PCR-RFLP）检测急性脑梗死患者（脑梗死组，128例）和健康体检者（正常对照组，111例） GPx-1基因C594T的基因型和等位基因频率。根据是否存在急性脑梗死的危险因素（高龄，高血压病，2型糖尿病，吸烟史）将脑梗死组分为8个亚组，计算各亚组急性脑梗死的OR值，并与正常对照组比较。结果急性脑梗死组患者GPx-1基因C594 T的基因型和等位基因频率与正常对照组相比差异无统计学意义（P＝0.24，P＝0.28）；经分层分析后，脑梗死组各亚组急性脑梗死的OR值与正常对照组相比差异无统计学意义。结论 GPx-1基因C594T多态性与急性脑梗死的发生无相关性。急性脑梗死的相关危险因素（高龄，高血压病，2型糖尿病，吸烟史）与GPx-1 C594T基因多态性共同作用时，对急性脑梗死的发生影响不显著。“,”Objective To explore the association between the C 594T polymorphism in Glutathione peroxidase-1 (GPx-1) and acute cerebral infarction.Methods The genotype and allele frequency in GPx-1 gene C594T was analyzed by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) among acute cerebral infarction patients ( cerebral infarction group , n=128 ) and healthy examinationers ( normal control group , n=111 ) . Patients were divided into eight subgroups according to risk factors ( age, hypertension, type 2 diabetes and smoking).Then calculated acute cerebral infarction relative OR value and compared with normal control group . Results There was no statistical significance of genotype and allele in GPx-1 gene C594T between cerebral infarction group and normal control group (P=0.24,P=0.28).In stratified analysis, the acute cerebral infarction relative OR value in each subgroups had no statistical differences with those in normal control group .Conclusion No significant correlation is observed between the GPx-1 gene C594T polymorphism and the incidence of acute cerebral infarction . When the GPx-1 gene C594T polymorphism and risk factors(age, hypertension, type 2 diabetes, and smoking) occur at the same time, there is no significant relevance to the incidence of acute cerebral infarction .