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过去两年来,甲型肝炎病毒(HAV)已能在几种细胞系统上繁殖。但现有能培养HAV的细胞对生产人用病毒疫苗来说都不理想。本文报告,HAV可适应于人细胞,也许是研究人用甲型肝炎疫苗的一个有用的宿主系统。作者取10～25周龄胎儿的肾和肺制备人肾细胞(HKC)和人成纤维细胞株(HFS)。HKC至少可传5代,HFS可传50多代。用含HAV的粪便悬液接种HKC传第一代,用放射免疫试验(RIA)检查,接种后第5周上清液初次出现HAV阳性。用最初出现HAV阳性的上一代的上清液作为在HKC上传下一代的接种物,以选出生长更快的病毒。传至第9代,用RIA检查,上清液最初出现HAV
Over the past two years, Hepatitis A virus (HAV) has been able to multiply in several cell systems. However, the existing cells capable of culturing HAV are not ideal for the production of human viral vaccines. This paper reports that HAV can be adapted to human cells and may be a useful host system for studying human Hepatitis A vaccines. The authors used kidney and lung preparations of human renal cells (HKC) and human fibroblast cell lines (HFS) from 10 to 25 weeks old fetuses. HKC can pass at least 5 generations, HFS can pass more than 50 generations. The first passage of HKC was inoculated with HAV-containing faecal suspension and examined by radioimmunoassay (RIA). The supernatants showed HAV positive for the first time after 5 weeks. The supernatants of the previous generation that initially appeared positive for HAV were used as an inoculum for uploading the next generation to HKC to select for the faster growing virus. At 9th passage, HAV was initially detected in the supernatants by RIA