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目的:研究牙科用钴铬钼合金对L929细胞生物学行为的影响。方法:制备医用纯钛、钴铬钼合金、钴铬合金、镍铬合金的浸提液,在浸提液与L929细胞作用24、48、72 h后,用倒置相差显微镜观察细胞的生长情况,并通过CCK-8实验评定4种材料的细胞毒性;采用流式细胞仪Annexin V-FITC/PI双染法评价材料对细胞凋亡的影响;应用吖啶橙染色法检测L929细胞在材料表面的黏附情况;采用SPSS17.0软件包对数据进行统计学分析。结果:倒置相差显微镜下观察,各时间点除镍铬合金组可见少量细胞核固缩,呈现轻微中毒状态外,其余3组细胞生长情况良好;钴铬钼合金组在各时间点的OD值、细胞凋亡率、细胞黏附数量均显著低于医用纯钛组(P<0.05),而高于钴铬合金组(P<0.05)和镍铬合金组(P<0.05);观察期内,医用纯钛组、钴铬钼合金组、钴铬合金组细胞毒性均为1级,镍铬合金组为2级,表现为轻微毒性。结论:钴铬钼合金对L929细胞的生物学行为无明显不良影响,符合口腔生物材料的临床应用要求,具有良好的生物相容性。
Objective: To study the effects of dental cobalt-chromium-molybdenum alloys on the biological behavior of L929 cells. Methods: The pure titanium, cobalt-chromium-molybdenum alloy, cobalt-chromium alloy and nickel-chromium alloy were prepared. After treated with L929 cells for 24,48 and 72 h, the growth of the cells was observed by inverted phase contrast microscope. The cytotoxicity of four materials was evaluated by CCK-8 assay. The effect of materials on apoptosis was evaluated by flow cytometry Annexin V-FITC / PI double staining. Acridine orange staining was used to detect the cytotoxicity of L929 cells on the surface of the material Adhesion conditions; using SPSS17.0 software package for statistical analysis of the data. Results: Under inverted phase contrast microscopy, except for the Ni-Cr alloy group, a small amount of nucleus pyknosis was observed at each time point, showing slight poisoning. The other three groups grew well. The OD value of the Co-Cr-Mo alloy group at each time point, (P <0.05), but higher than that of cobalt-chromium alloy (P <0.05) and nickel-chromium alloy group (P <0.05). During the observation period, The cytotoxicity of titanium group, cobalt-chromium-molybdenum alloy group, cobalt-chromium alloy group was grade 1, and that of nichrome group was grade 2, showing slight toxicity. CONCLUSION: Cobalt-chromium-molybdenum alloy has no obvious adverse effect on the biological behavior of L929 cells, which meets the clinical application requirements of oral biomaterials and has good biocompatibility.