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目的：研究自身失活型（SIN）慢病毒载体感染脐带血造血干细胞的方法及效率。方法利用钙转的方法实现携带有绿色荧光蛋白（GFP）的SIN慢病毒载体的包装，将浓缩后的病毒按照一定的感染复数（约为175）感染人脐带血CD34＋细胞24 h，换正常培养基继续培养2 d后，利用流式细胞学方法检测CD34＋％及CD34＋GFP＋％，并对感染SIN慢病毒载体的CD34＋细胞进行集落形成能力检测。结果 SIN慢病毒载体感染CD34＋细胞24 h后，换正常培养基继续培养2 d，检测细胞CD34＋％可维持在97．80％，且感染效率可达34．20％；对比正常的脐带血CD34＋细胞及经SIN慢病毒载体感染后的CD34＋细胞，二者的集落形成能力差异无统计学意义（P＞0．05）。结论通过上述方法，在不影响脐带血CD34＋细胞体外分化能力的前提下，实现了SIN慢病毒载体对CD34＋细胞的有效感染。“,”Objective To identify the methods and efficiency of self-inactivating (SIN) lentivirus vector transfection in human cord blood stem cells. Methods SIN lentivirus vector was packaged by calcium phosphate transfection method and concentrated by ultracentrifugation to achieve titers of 107/ml. After pre-stimulating , the human cord blood CD34+cells were transfected for 24 h in diluted lentivirus vector supernatant (MOI=175) and then cultured for 2 more days in the serum-free media with cytokines. The cells were collected and detected by Flow cytometer for CD34+%and CD34+GFP+%at day2, and then the CD34+cells were isolated and tested in colony-forming assay. Results At day2, the CD34+cells was up to 97.80%, and the CD34+GFP+cells was 34.20%. Furthermore, there was no difference in the cell differentiation ability between the control group (CD34+cells with no transfection) and the experiment group (CD34+cells transfected with SIN lentivirus vector). Conclusion The cord blood CD34+cells can be transfected effectively with this method. Besides, the ability of cell differentiation in vitro was not significantly affected by the SIN lentivirus vector.