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本文试图证明在小鼠皮肤癌发生中免疫机制的作用．应用不同次数促癌剂ＴＰＡ后，用Ｓ．Ｄ大鼠脾细胞免疫昆明种雌性小鼠，２周后在小鼠足部进行免疫攻击引出全身性延迟型过敏反应（ＳｙｓｔｅｍｉｃＤｅｌａｙｅｄＴｙｐｅＨｙｐｅｒｓｅｎｓｉｔｉｖｉｔｙ，ＳＤＴＨ）．以攻击前后足容积差和足伊文思兰提取物光密度值大小为指标，观察ＤＴＨ强度的改变．结果表明，随着ＴＰＡ促癌次数的增加，足容积差和光密度值均减少，显示促癌剂ＴＰＡ对ＳＤＴＨ的抑制作用并表现出一定的量效关系，促癌剂应用４周（３μｇ／０．２ｍＬ，８次）后所出现的免疫抑制与实验小鼠第５周开始出现乳头瘤密切相关，此外本文还证明应用不同次数促癌剂ＴＰＡ后，免疫器官脾的相对重量呈现下降趋势
This article attempts to demonstrate the role of immune mechanisms in the development of skin cancer in mice. After applying different times of TPA, use S. D rat spleen cells were immunized with Kunming female mice, and immunostained in the foot of mice two weeks later led to Systemic Delayed Type Hypersensitivity (SDTH). The changes of DTH intensity were observed with the difference of foot volume before and after challenge and the value of optical density of Evans blue extract. The results showed that with the increase of TPA, the foot volume difference and the optical density value decreased, indicating that the TPA had a dose-dependent effect on the inhibition of SDTH. After 4 weeks (3μg / 0 .2mL, 8 times) appeared immunosuppression and experimental mice began to appear in the fifth week of papilloma, in addition, this paper also demonstrated that the application of different doses of TPA, immune organs, the relative weight of the spleen showed a downward trend