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从全叶蛋白比较入手，研究了温敏失绿突变体水稻（ＯｒｙｚａｓａｔｉｖａＬ．）１１０３ｓ失绿前、后的变化，并结合该突变体在不同温度处理和遗传背景下叶片全蛋白的变化特征分析了变温诱导与失绿的关系。结果表明，诱导后１１０３ｓ的叶片上，失绿部分的组织中没有出现冷胁迫的迹象，其Ｒｕｂｉｓｃｏ的大、小亚基表现正常。一个５１ｋＤ（ＰＩ＝４．５）的特异蛋白Ｐ１在失绿部分的组织中消失，而在叶片绿色部分的组织中检测到了Ｐ１蛋白，不过量有所减少，说明在失绿叶片上突变基因的表达存在组织差异性。Ｐ１蛋白在常温下生长的１１０３ｓ叶片中为一大量蛋白，此蛋白在“８９０２ｓ”、“窄叶青８号”等品种中均可检测到；并且持续低温处理的１１０３ｓ植株和１１０３ｓ×８９０２ｓ杂交一代的叶片中Ｐ１蛋白的表达未受影响。由此推测，Ｐ１蛋白是水稻叶片中的一个与叶绿素的代谢过程密切相关的重要功能蛋白。在１１０３ｓ中，Ｐ１蛋白的变化不是温度诱导的直接后果，而是受突变所导致的温敏过程调控的下游变化。
Starting from the comparison of the whole leaf protein, we studied the changes of green-sensitive mutant Oryzasativa L. 1103s before and after chlorosis, and analyzed the changes of total proteins in the leaves under different temperature treatments and genetic background Relationship between temperature induction and chlorosis. The results showed that there was no evidence of cold stress in the tissues of the chloridized leaves at 1103s after induction, and the large and small subunits of Rubisco showed normal. A 51 kD (PI = 4.5) specific protein P1 disappeared in the green-stained tissue whereas P1 protein was detected in the green part of the stalk, with a slight decrease, indicating that the mutant gene was expressed on chlorotic leaf There are organizational differences. The P1 protein was a large number of proteins in 1103s leaves grown at room temperature. The protein was detected in “8902s” and “Narrow Yeqing 8”. The 1103s and 1103s × 8902s The expression of P1 protein in leaves was unaffected. Therefore, it is speculated that P1 protein is an important functional protein in rice leaf which is closely related to the metabolic process of chlorophyll. In 1103s, the change of P1 protein is not a direct consequence of temperature induction but a downstream change regulated by the temperature-sensitive process caused by the mutation.