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胃康宁具有健脾化痰、清热解毒、理气活血、消瘀散结功效。中药在临床上治疗胃癌有一定疗效,为此,我们进行了动物实验,研究胃康宁对大鼠实验性胃癌的防治作用,以及对血管内皮生长因子(VEGF)表达的影响。 1 材料和方法 1.1 材料 ①中药胃康宁制备:由党参、半枝莲、五灵脂等组成,功效:健脾化痰、清热解毒、理气活血、消瘀散结,制成生药(g):药液(mL)=1(g):1(mL)浓度的制剂,加防腐剂苯甲酸(0.1g/100mL)、4℃冷藏保存,供大鼠灌胃之用。②实验动物:(?)Wistar大鼠50只,2~3月龄,体质量120g~140g,清洁级,中山医科大学实验动物中心提供(广东省实验动物监测所检证
Wei Kang Ning has the effect of invigorating spleen and removing sputum, clearing heat and detoxicating, regulating qi and activating blood circulation, eliminating sputum and dispelling sputum. Traditional Chinese medicine has a certain curative effect in the treatment of gastric cancer in clinical practice. Therefore, we conducted animal experiments to study the effect of Wei Kang Ning on the prevention and treatment of experimental gastric cancer in rats and the effect on the expression of vascular endothelial growth factor (VEGF). 1 Materials and Methods 1.1 Materials 1 Preparation of Traditional Chinese Medicine WeiGangNing: Made of Codonopsis pilosula, Scutellaria barbata, Wulingzhi, etc. Efficacy: Jianpi Huayu, Qingrejiedu, Qiqihuoxue, Xiaoyu Sanjie, made of crude drugs (g): Liquid medicine (mL) = 1 (g): 1 (mL) concentration of the preparation, plus preservative benzoic acid (0.1g/100mL), cryopreserved at 4 °C, for rat gavage use. 2 Experimental animals: (?) Wistar rats 50, 2 to 3 months old, body weight 120g ~ 140g, clean grade, provided by the Laboratory Animal Center of Sun Yat-sen University of Medical Sciences (Guangdong Provincial Laboratory Animal Inspection Laboratory