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目的 探讨扶正化瘀方防治慢性肝病的作用机制及药效学配伍意义．方法 将扶正化瘀方分为扶正组、化瘀组、虫草组、丹参组及丹参加虫草组，灌胃给药分离药物血清，温育传一代大鼠肝星状细胞和原代肝细胞． 半定量ＲＴＰＣＲ 法检测Ⅰ型胶原ｍ ＲＮＡ表达，ＥＬＩＳＡ 法检测Ⅰ型胶原和清蛋白分泌，３ ＨＴｄＲ 和３ Ｈ脯氨酸掺入分别观察细胞增殖和活力．结果 各组药物均可抑制星状细胞的增殖，其中化瘀药效果最明显；各组药物均可抑制星状细胞Ⅰ型胶原ｍ ＲＮＡ 和蛋白的生成，以扶正组效果最佳；全方明显促进肝细胞和肝星状细胞蛋白质生成，而各拆方组均无此作用．结论 扶正与化瘀两法配伍组成扶正化瘀方在防治慢性肝病方面具有整体优势．
Objective To explore the mechanism and pharmacodynamic compatibility of Fuzheng Huayu Decoction in preventing and treating chronic liver diseases. Methods Fuzheng Huayu Decoction was divided into Fuzheng group, Huayu group, Cordyceps group, Salvia miltiorrhiza group and Danjiao Cordyceps group. Gastric stellate cells and primary hepatocytes were cultured for one generation after intragastric administration. Semi-quantitative RT-PCR was used to detect the expression of type I collagen m RNA. ELISA was used to detect the secretion of type I collagen and albumin. 3 H-TdR and 3 H-proline incorporation were used to observe cell proliferation and viability, respectively. Results All groups of drugs could inhibit the proliferation of stellate cells, among which the effect of phlegm-resolving drugs was the most obvious; all groups of drugs could inhibit the production of type I collagen m RNA and protein in stellate cells, and the effect of the best in Fuzheng group was obvious; Promote hepatocyte and hepatic stellate cell protein production, and the split group did not have this effect. Conclusion The combination of Fuzheng and Huatan combined to form a Fuzheng Huayufang has overall advantages in the prevention and treatment of chronic liver diseases.