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受52及103mC/kg照射的绵羊淋巴细胞中出现3H-TdR(税氧胸腺嘧啶核苷)自发掺入DNA增加,受照动物淋巴细胞中自发DNA合成活性改变伴随外周血淋巴细胞中总蛋白量的增加.实验用18只茨冈种母绵羊,γ射线照射31、52及103mC/kg,非照射绵羊淋巴细胞DNA中~3H-TdR自发掺入量为200～1200cpm/10~6细胞.动物适应饲养环境2周后,淋巴细胞DNA中~3H-TdR自发掺入量为428cpm/106细胞,之后则无变化.照射52mC/kg时,绵羊淋巴细胞DNA中~3H-TdR自发掺入量在第5天提高到几乎5倍,7～10天增加到2～3倍,然而受103mC/kg照射动物该指标在照后1～7天增高约5～10倍.还发现52及103mC/kg照射母绵羊淋巴细胞中,总蛋白含量增加,但增加的高峰发生在绵羊淋巴细胞DNA中.~3H-TdR自发掺入量最大增长期以后.因此,照射诱导的淋巴细胞DNA中~3H-TdR自然掺入量增高,不仅是外周血淋巴细胞
3H-TdR (tax-thymidine) spontaneous incorporation of DNA into sheep lymphocytes irradiated at 52 and 103 mC / kg increased spontaneously DNA synthesis activity in irradiated animal lymphocytes accompanied by changes in the amount of total protein in peripheral blood lymphocytes .In the experiment, 18 Ibzeen female sheep were irradiated with γ ray at 31, 52 and 103 mC / kg respectively, and the ~ 3H-TdR incorporation in non-irradiated sheep lymphocytes was 200 ~ 1200cpm / 10 ~ 6 spontaneously. After 2 weeks of feeding, the ~ 3H-TdR incorporation in lymphocyte DNA was 428 cpm / 106 cells spontaneously, and then there was no change.When irradiated with 52mC / kg, the ~ 3H-TdR spontaneous incorporation in sheep’s lymphocyte DNA was 5 days increased to almost 5 times, 7 to 10 days increased to 2 to 3 times, but by 103mC / kg irradiation of animals this index 1 to 7 days after irradiation increased about 5 to 10 times also found 52 and 103mC / kg irradiation The total protein content of the female sheep lymphocytes increased, but the increased peak occurred in the sheep’s lymphocytes DNA ~ 3H-TdR spontaneous incorporation after the maximum growth period.Therefore, irradiation-induced lymphocyte DNA ~ 3H-TdR naturally Incorporation increased, not only peripheral blood lymphocytes