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目的糖尿病(Diabetes Mellitus,DM)是冠心病发病的常见危险因素之一,糖尿病肾病(Diabetic Nephropathy,DN)是冠心病最常见并发症。通过检测大鼠肾脏形态学结构改变以及蛋白激酶C(protein kinase C,PKC)表达来揭示中药黄芩苷胶囊对糖尿病肾病的影响。方法 SD雄性大鼠70只,SPF级,随机分成正常组10只,剩余60只采用高脂饲料1月+单次腹腔注射链脲佐菌素(streptozotocin,STZ)(35 mg/kg)进行DM造模。1周后测得大鼠空腹血糖≥16.7 mmol/L为DM模型成功。DM成模大鼠给予自由饮食,当第8周末,大鼠尿蛋白出现阳性则DN模型成功。成模DN大鼠随机分成模型组、黄芩苷组、依那普利组。分别以相应药物灌胃,给药6周末,所有大鼠称重测血糖,麻醉后断头处死,取出左肾,放入10%中性甲醛液中固定。结果与正常组比较,模型组大鼠血糖和肾指数都明显升高,差异有统计学意义(P<0.05);肾小球体积增大,肾球囊腔比例失调,肾小管上皮细胞水肿,脱落,肾小管上皮细胞PKC表达增加。与模型组比较,黄芩苷组和依那普利组血糖都明显降低,差异有统计学意义(P<0.05),且黄芩苷组肾指数下降,差异有统计学意义(P<0.05);肾脏病变均减轻,PKC表达均减少,且黄芩苷组优于依那普利组。结论中药黄芩苷胶囊能减轻早期DN大鼠肾脏损伤,其机制可能与它下调PKC表达有关,对减轻DM的危害与预防冠心病的发病可能具有一定临床价值。
Diabetes mellitus (DM) is one of the common risk factors of coronary heart disease. Diabetic Nephropathy (DN) is the most common complication of coronary heart disease. The effect of baicalin capsules on diabetic nephropathy was revealed by examining the morphological changes in rat kidneys and the expression of protein kinase C (PKC). Methods Seventy male Sprague-Dawley rats were randomly divided into normal control group (n = 10) and SPF group (n = 10). The remaining 60 rats were treated with high-fat diet for 1 month and single intraperitoneal injection of streptozotocin (STZ) Modeling. One week later, the fasting blood glucose> 16.7 mmol / L in rats was determined as DM model success. DM rats were given a free diet. At the end of the 8th week, DN rats were successfully treated with positive urine protein. The model rats were randomly divided into model group, baicalin group and enalapril group. Respectively, the corresponding drug gavage, 6 weeks after administration, all rats were weighed to measure blood glucose, decapitated after anesthesia, remove the left kidney, placed in 10% formaldehyde solution fixed. Results Compared with the normal group, the blood glucose and renal index in the model group were significantly increased (P <0.05), the glomerular volume was increased, the proportion of the renal balloon cavity was imbalanced, the tubular epithelial cell edema, Shedding, tubular epithelial PKC expression increased. Compared with the model group, the blood glucose in the baicalin group and the enalapril group were significantly lower, the difference was statistically significant (P <0.05), and the renal index in the baicalin group decreased, the difference was statistically significant (P <0.05); kidney Lesions were reduced, PKC expression decreased, and the baicalin group is better than the enalapril group. Conclusion Baicalin capsule can reduce renal damage in early DN rats. The mechanism may be related to its down-regulation of PKC expression. It may have some clinical value in reducing the damage of DM and preventing the occurrence of coronary heart disease.