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目的采用固相萃取-气相色谱法检测全血中佐匹克隆。方法采用Oasis HLB固相萃取柱对样品进行前处理,去离子水、0.5%氨水-甲醇/水((V/V 40∶60)溶液先后淋洗,二氯甲烷/异丙醇(V/V 75∶25)洗脱后进行GC/NPD检测。结果血液中佐匹克隆在50～5 000ng/mL范围内线性良好(R2=0.998 8),平均萃取回收率为96.9%,检出限为30ng/mL,日内RSD为2.1%～5.7%,日间RSD为3.3%～6.2%,结论固相萃取-气相色谱检测法灵敏度高,重现性好,可在血液中佐匹克隆的检测中选用。
Objective To detect zopiclone in whole blood by solid-phase extraction and gas chromatography. Methods Oasis HLB solid phase extraction column was used to pretreat the samples with deionized water, then with 0.5% aqueous ammonia / methanol / water (V / V 40:60) solution and then with dichloromethane / isopropanol 75:25) .RESULTS The zopiclone in blood was linear in the range of 50-5 000 ng / mL (R2 = 0.998 8) with an average extraction recovery of 96.9% with a detection limit of 30 ng / mL, intraday RSD was 2.1% ~ 5.7%, daytime RSD was 3.3% ~ 6.2%. Conclusion Solid phase extraction - gas chromatography has the advantages of high sensitivity, good reproducibility and can be used in the detection of zopiclone in the blood.