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本文应用生物素标记重组人α_1 型干扰素(rHuIFN-α_1)工程菌的总DNA作为探针,检测rHuIFN-α_1制品中的外源DNA,其灵敏度可达10pg。用蛋白酶K(Proteinase K)消化制品中的蛋白,排除了使用生物素所常见的非特异性影响。
In this paper, the total DNA of recombinant human α 1-interferon (rHuIFN-α_1) engineered bacteria was used as a probe to detect the foreign DNA in rHuIFN-α 1 product with a sensitivity of 10 pg. Digestion of the protein in the preparation with Proteinase K precludes the non-specific effects commonly seen with biotin.