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目的了解深圳地区耐甲氧西林金黄色葡萄球菌(MRSA)分子型别特征及其对常用抗生素的耐药情况,为治疗和防控MRSA感染提供科学依据。方法对2012-2014年深圳地区9家哨点医院临床分离的228株MRSA进行多位点序列分型,检测其对庆大霉素(GEN)、环丙沙星(CIP)、磺胺甲噁唑/甲氧苄啶(SXT)、红霉素(ERY)、万古霉素(VAN)、克林霉素(CLI)、四环素(TET)和利奈唑胺(LNZ)等8种非-β内酰胺类抗生素的敏感性。结果 228株MRSA可分为33种ST型,其中以ST59型为主,占41.7%(95/228);其次为ST338型,占15.4%(35/228);经e BURST软件分析它们属于15种克隆复合体和1个单一克隆,其中CC59为主要克隆,占61.0%(139/228),其次为CC239,占9.6%(22/228);ST59和ST338同属于CC59,耐药谱均为ERY/CLI/TET。结论深圳地区流行的MRSA以CC59为主,可选用环丙沙星或磺胺甲噁唑/甲氧苄啶治疗。
Objective To understand the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) in Shenzhen and their resistance to commonly used antibiotics to provide a scientific basis for the treatment and prevention of MRSA infection. Methods A total of 228 strains of MRSA clinically isolated from 9 sentinel hospitals in Shenzhen area from 2012 to 2014 were genotyped by multiple loci. The genotypes of gentamicin (GEN), ciprofloxacin (CIP), sulfamethoxazole 8 kinds of non-β-lactamases such as trimethoprim (SXT), erythromycin (ERY), vancomycin (VAN), clindamycin (CLI), tetracycline (TET) and linezolid (LNZ) Class antibiotic sensitivity. Results 228 MRSA strains were divided into 33 ST types, of which ST59 type accounted for 41.7% (95/228), followed by ST338 type (15.4% (35/228)). According to eURBURST software, they were 15 (CC59) accounted for 61.0% (139/228) followed by CC239, accounting for 9.6% (22/228). Both ST59 and ST338 belonged to CC59 and the resistant spectrum was ERY / CLI / TET. Conclusion The prevalence of MRSA in Shenzhen is mainly CC59, which may be treated with ciprofloxacin or sulfamethoxazole / trimethoprim.